Protective roles of autophagy in retinal pigment epithelium under high glucose condition via regulating PINK1/Parkin pathway and BNIP3L

Abstract Background Our study aimed to investigate the roles of autophagy against high glucose induced response in retinal pigment epithelium (ARPE-19 cells). Methods The morphological changes and reactive oxygen species (ROS) generation in ARPE-19 cells under high glucose treatment were respect...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Huang,Chengchi, Lu,Hong, Xu,Junyu, Yu,Hongmin, Wang,Xiaodan, Zhang,Xiaomei
Lenguaje:	English
Publicado:	Sociedad de Biología de Chile 2018
Materias:	Diabetic retinopathy Retinal pigment epithelium Autophagy High glucose
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100219
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0716-97602018000100219
record_format	dspace
spelling	oai:scielo:S0716-976020180001002192018-08-20Protective roles of autophagy in retinal pigment epithelium under high glucose condition via regulating PINK1/Parkin pathway and BNIP3LHuang,ChengchiLu,HongXu,JunyuYu,HongminWang,XiaodanZhang,Xiaomei Diabetic retinopathy Retinal pigment epithelium Autophagy High glucose Abstract Background Our study aimed to investigate the roles of autophagy against high glucose induced response in retinal pigment epithelium (ARPE-19 cells). Methods The morphological changes and reactive oxygen species (ROS) generation in ARPE-19 cells under high glucose treatment were respectively detected using the transmission electron microscopy and flow cytometry. The expression levels of Parkin, PINK1, BNIP3L, LC3-I and LC3-II in ARPE-19 cells received high glucose treatment were measured by western blot after pretreatment of carbonyl cyanide m-chlorophenylhydrazone (CCCP), 3-methyladenine (3-MA), N-acetyl cysteine (NAC) or cyclosporin A (CsA) followed by high glucose treatment. Results ARPE-19 cells subjected to high glucose stress showed an obvious reduction in the LC3-I expression and significant increase in the number of autophagosomes, in the intracellular ROS level, and in the expression levels of Parkin, PINK1, BNIP3L and LC3-II (p < 0.05). Pretreatment with CCCP significantly reduced the LC3-I expression and increased the expression levels of Parkin, PINK1, BNIP3L and LC3-II (p < 0.05). ARPE-19 cells pretreated with CsA under high glucose stress showed markedly down-regulated expressions of Parkin, PINK1 and BNIP3L compared with the cells treated with high glucose (p < 0.05). Pretreatment of ARPE-19 cells with NAC or 3-MA under high glucose stress resulted in a marked reduction in the expression levels of PINK1, BNIP3L and LC3-II (p < 0.05). Meanwhile, the expression level of Parkin in the ARPE-19 cells pretreated with NAC under high glucose stress was comparable with that in the control cells. Conclusion Autophagy might have protective roles against high glucose induced injury in ARPE19 cells via regulating PINK1/Parkin pathway and BNIP3L.info:eu-repo/semantics/openAccessSociedad de Biología de ChileBiological Research v.51 20182018-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100219en10.1186/s40659-018-0169-4
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Diabetic retinopathy Retinal pigment epithelium Autophagy High glucose
spellingShingle	Diabetic retinopathy Retinal pigment epithelium Autophagy High glucose Huang,Chengchi Lu,Hong Xu,Junyu Yu,Hongmin Wang,Xiaodan Zhang,Xiaomei Protective roles of autophagy in retinal pigment epithelium under high glucose condition via regulating PINK1/Parkin pathway and BNIP3L
description	Abstract Background Our study aimed to investigate the roles of autophagy against high glucose induced response in retinal pigment epithelium (ARPE-19 cells). Methods The morphological changes and reactive oxygen species (ROS) generation in ARPE-19 cells under high glucose treatment were respectively detected using the transmission electron microscopy and flow cytometry. The expression levels of Parkin, PINK1, BNIP3L, LC3-I and LC3-II in ARPE-19 cells received high glucose treatment were measured by western blot after pretreatment of carbonyl cyanide m-chlorophenylhydrazone (CCCP), 3-methyladenine (3-MA), N-acetyl cysteine (NAC) or cyclosporin A (CsA) followed by high glucose treatment. Results ARPE-19 cells subjected to high glucose stress showed an obvious reduction in the LC3-I expression and significant increase in the number of autophagosomes, in the intracellular ROS level, and in the expression levels of Parkin, PINK1, BNIP3L and LC3-II (p < 0.05). Pretreatment with CCCP significantly reduced the LC3-I expression and increased the expression levels of Parkin, PINK1, BNIP3L and LC3-II (p < 0.05). ARPE-19 cells pretreated with CsA under high glucose stress showed markedly down-regulated expressions of Parkin, PINK1 and BNIP3L compared with the cells treated with high glucose (p < 0.05). Pretreatment of ARPE-19 cells with NAC or 3-MA under high glucose stress resulted in a marked reduction in the expression levels of PINK1, BNIP3L and LC3-II (p < 0.05). Meanwhile, the expression level of Parkin in the ARPE-19 cells pretreated with NAC under high glucose stress was comparable with that in the control cells. Conclusion Autophagy might have protective roles against high glucose induced injury in ARPE19 cells via regulating PINK1/Parkin pathway and BNIP3L.
author	Huang,Chengchi Lu,Hong Xu,Junyu Yu,Hongmin Wang,Xiaodan Zhang,Xiaomei
author_facet	Huang,Chengchi Lu,Hong Xu,Junyu Yu,Hongmin Wang,Xiaodan Zhang,Xiaomei
author_sort	Huang,Chengchi
title	Protective roles of autophagy in retinal pigment epithelium under high glucose condition via regulating PINK1/Parkin pathway and BNIP3L
title_short	Protective roles of autophagy in retinal pigment epithelium under high glucose condition via regulating PINK1/Parkin pathway and BNIP3L
title_full	Protective roles of autophagy in retinal pigment epithelium under high glucose condition via regulating PINK1/Parkin pathway and BNIP3L
title_fullStr	Protective roles of autophagy in retinal pigment epithelium under high glucose condition via regulating PINK1/Parkin pathway and BNIP3L
title_full_unstemmed	Protective roles of autophagy in retinal pigment epithelium under high glucose condition via regulating PINK1/Parkin pathway and BNIP3L
title_sort	protective roles of autophagy in retinal pigment epithelium under high glucose condition via regulating pink1/parkin pathway and bnip3l
publisher	Sociedad de Biología de Chile
publishDate	2018
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602018000100219
work_keys_str_mv	AT huangchengchi protectiverolesofautophagyinretinalpigmentepitheliumunderhighglucoseconditionviaregulatingpink1parkinpathwayandbnip3l AT luhong protectiverolesofautophagyinretinalpigmentepitheliumunderhighglucoseconditionviaregulatingpink1parkinpathwayandbnip3l AT xujunyu protectiverolesofautophagyinretinalpigmentepitheliumunderhighglucoseconditionviaregulatingpink1parkinpathwayandbnip3l AT yuhongmin protectiverolesofautophagyinretinalpigmentepitheliumunderhighglucoseconditionviaregulatingpink1parkinpathwayandbnip3l AT wangxiaodan protectiverolesofautophagyinretinalpigmentepitheliumunderhighglucoseconditionviaregulatingpink1parkinpathwayandbnip3l AT zhangxiaomei protectiverolesofautophagyinretinalpigmentepitheliumunderhighglucoseconditionviaregulatingpink1parkinpathwayandbnip3l
_version_	1718441572611129344

Protective roles of autophagy in retinal pigment epithelium under high glucose condition via regulating PINK1/Parkin pathway and BNIP3L

Ejemplares similares