Lipopolyamine-mediated transfection of reporter plasmids into a fish cell line

Conditions have been optimised to transfect the fish cell line CHSE-214 to measure expression, maintenance and putative chromosomal integration of the reporter gene LUC, spliced into two versions of an expression vector. The first is pCMVL, and the second p103, a novel pCMVL-derived plasmid to which...

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Autores principales: Villalobos,Patricio, Rojas,M. Verónica, Conejeros,Pablo, Marshall,Sergio H.
Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso 1999
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34581999000200005
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