An RT-PCR artifact in the characterization of bacterial operons

During the course of experiments involving RT-PCR to investigate the co-transcription of the pet and res operons of the bacterium Acidithiobacillus ferrooxidans we observed that the mRNA of certain gene pairs can be amplified by RT-PCR in the absence of added RT-primer. We provide experimental evide...

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Autores principales: Guacucano,Maritza, Levican,Gloria, S. Holmes,David, Jedlicki,Eugenia
Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso 2000
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582000000300005
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