An RT-PCR artifact in the characterization of bacterial operons
During the course of experiments involving RT-PCR to investigate the co-transcription of the pet and res operons of the bacterium Acidithiobacillus ferrooxidans we observed that the mRNA of certain gene pairs can be amplified by RT-PCR in the absence of added RT-primer. We provide experimental evide...
Guardado en:
Autores principales: | Guacucano,Maritza, Levican,Gloria, S. Holmes,David, Jedlicki,Eugenia |
---|---|
Lenguaje: | English |
Publicado: |
Pontificia Universidad Católica de Valparaíso
2000
|
Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582000000300005 |
Etiquetas: |
Agregar Etiqueta
Sin Etiquetas, Sea el primero en etiquetar este registro!
|
Ejemplares similares
-
Comparison of RT-PCR, RT-nested PCRs, and real-time PCR for diagnosis of severe fever with thrombocytopenia syndrome: a prospective study
por: Sehrish Jalal, et al.
Publicado: (2021) -
Covid-19 diagnosis by combining RT-PCR and pseudo-convolutional machines to characterize virus sequences
por: Juliana Carneiro Gomes, et al.
Publicado: (2021) -
Detecting operons in bacterial genomes via visual representation learning
por: Rida Assaf, et al.
Publicado: (2021) -
Unprecedented High-Resolution View of Bacterial Operon Architecture Revealed by RNA Sequencing
por: Tyrrell Conway, et al.
Publicado: (2014) -
Interplay of gene expression noise and ultrasensitive dynamics affects bacterial operon organization.
por: J Christian J Ray, et al.
Publicado: (2012)