An RT-PCR artifact in the characterization of bacterial operons
During the course of experiments involving RT-PCR to investigate the co-transcription of the pet and res operons of the bacterium Acidithiobacillus ferrooxidans we observed that the mRNA of certain gene pairs can be amplified by RT-PCR in the absence of added RT-primer. We provide experimental evide...
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| Auteurs principaux: | , , , |
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| Langue: | English |
| Publié: |
Pontificia Universidad Católica de Valparaíso
2000
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| Accès en ligne: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582000000300005 |
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