Improved affinity selection using phage display technology and off-rate based selection
Flow systems such as a BIAcore biosensor can be very efficient tools to isolate high affinity antibody fragments from affinity matured phage display libraries. Here we show that using flow based selection, we can readily isolate a variant with a 35-fold higher affinity, especially with a 7 fold bett...
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| Auteurs principaux: | , , , |
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| Langue: | English |
| Publié: |
Pontificia Universidad Católica de Valparaíso
2006
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| Accès en ligne: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000200011 |
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