Improved affinity selection using phage display technology and off-rate based selection

Flow systems such as a BIAcore biosensor can be very efficient tools to isolate high affinity antibody fragments from affinity matured phage display libraries. Here we show that using flow based selection, we can readily isolate a variant with a 35-fold higher affinity, especially with a 7 fold bett...

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Autores principales:	Yuan,Bin, Schulz,Philip, Liu,Ruitian, Sierks,Michael R.
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2006
Materias:	affinity maturation beta-amyloid (Abeta) dissociation rate phage display
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000200011
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spelling	oai:scielo:S0717-345820060002000112006-12-11Improved affinity selection using phage display technology and off-rate based selectionYuan,BinSchulz,PhilipLiu,RuitianSierks,Michael R. affinity maturation beta-amyloid (Abeta) dissociation rate phage display Flow systems such as a BIAcore biosensor can be very efficient tools to isolate high affinity antibody fragments from affinity matured phage display libraries. Here we show that using flow based selection, we can readily isolate a variant with a 35-fold higher affinity, especially with a 7 fold better off-rate, compared to the parent clone after only a single round of selection from a second generation affinity matured phage display library. The flow system represents a fast method to isolate affinity improved antibody fragments and can be particularly useful for isolating antibodies to antigens that have poor solubility, are toxic to the host cell, or prone to aggregation.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.9 n.2 20062006-04-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000200011en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	affinity maturation beta-amyloid (Abeta) dissociation rate phage display
spellingShingle	affinity maturation beta-amyloid (Abeta) dissociation rate phage display Yuan,Bin Schulz,Philip Liu,Ruitian Sierks,Michael R. Improved affinity selection using phage display technology and off-rate based selection
description	Flow systems such as a BIAcore biosensor can be very efficient tools to isolate high affinity antibody fragments from affinity matured phage display libraries. Here we show that using flow based selection, we can readily isolate a variant with a 35-fold higher affinity, especially with a 7 fold better off-rate, compared to the parent clone after only a single round of selection from a second generation affinity matured phage display library. The flow system represents a fast method to isolate affinity improved antibody fragments and can be particularly useful for isolating antibodies to antigens that have poor solubility, are toxic to the host cell, or prone to aggregation.
author	Yuan,Bin Schulz,Philip Liu,Ruitian Sierks,Michael R.
author_facet	Yuan,Bin Schulz,Philip Liu,Ruitian Sierks,Michael R.
author_sort	Yuan,Bin
title	Improved affinity selection using phage display technology and off-rate based selection
title_short	Improved affinity selection using phage display technology and off-rate based selection
title_full	Improved affinity selection using phage display technology and off-rate based selection
title_fullStr	Improved affinity selection using phage display technology and off-rate based selection
title_full_unstemmed	Improved affinity selection using phage display technology and off-rate based selection
title_sort	improved affinity selection using phage display technology and off-rate based selection
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000200011
work_keys_str_mv	AT yuanbin improvedaffinityselectionusingphagedisplaytechnologyandoffratebasedselection AT schulzphilip improvedaffinityselectionusingphagedisplaytechnologyandoffratebasedselection AT liuruitian improvedaffinityselectionusingphagedisplaytechnologyandoffratebasedselection AT sierksmichaelr improvedaffinityselectionusingphagedisplaytechnologyandoffratebasedselection
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Improved affinity selection using phage display technology and off-rate based selection

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