Production of Rhodotorula glutinis: a yeast that secretes alpha-L-arabinofuranosidase

Rhodotorula glutinis is a yeast that secretes the enzyme alpha-L-arabinofuranosidase (E.C. 3.2.1.55) into the culture medium and thus has an interesting biotechnological potential. To determine improved culture conditions of this organism, different factors of the culture media were evaluated such a...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Martínez,Claudio, Gertosio,Cecilia, Labbe,Angelica, Pérez,Rafael, Ganga,Maria Angélica
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2006
Materias:	alpha-L-arabinofuranosidase yeast enzymes wine
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000400009
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:scielo:S0717-34582006000400009
record_format	dspace
spelling	oai:scielo:S0717-345820060004000092007-01-12Production of Rhodotorula glutinis: a yeast that secretes alpha-L-arabinofuranosidaseMartínez,ClaudioGertosio,CeciliaLabbe,AngelicaPérez,RafaelGanga,Maria Angélica alpha-L-arabinofuranosidase yeast enzymes wine Rhodotorula glutinis is a yeast that secretes the enzyme alpha-L-arabinofuranosidase (E.C. 3.2.1.55) into the culture medium and thus has an interesting biotechnological potential. To determine improved culture conditions of this organism, different factors of the culture media were evaluated such as the use of peptone as nitrogen source, salts composition, pH and growth temperature. Likewise, beet molasses and beet cosette were tested as industrial carbon sources to induce the production of the enzyme and how they influence the yeast growth. Based on these studies a culture medium is proposed for growth of this yeast in a continuous system. By assaying different dilution rates an average specific activity for the enzyme of 82.4 U/mg of protein was obtained.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.9 n.4 20062006-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000400009en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	alpha-L-arabinofuranosidase yeast enzymes wine
spellingShingle	alpha-L-arabinofuranosidase yeast enzymes wine Martínez,Claudio Gertosio,Cecilia Labbe,Angelica Pérez,Rafael Ganga,Maria Angélica Production of Rhodotorula glutinis: a yeast that secretes alpha-L-arabinofuranosidase
description	Rhodotorula glutinis is a yeast that secretes the enzyme alpha-L-arabinofuranosidase (E.C. 3.2.1.55) into the culture medium and thus has an interesting biotechnological potential. To determine improved culture conditions of this organism, different factors of the culture media were evaluated such as the use of peptone as nitrogen source, salts composition, pH and growth temperature. Likewise, beet molasses and beet cosette were tested as industrial carbon sources to induce the production of the enzyme and how they influence the yeast growth. Based on these studies a culture medium is proposed for growth of this yeast in a continuous system. By assaying different dilution rates an average specific activity for the enzyme of 82.4 U/mg of protein was obtained.
author	Martínez,Claudio Gertosio,Cecilia Labbe,Angelica Pérez,Rafael Ganga,Maria Angélica
author_facet	Martínez,Claudio Gertosio,Cecilia Labbe,Angelica Pérez,Rafael Ganga,Maria Angélica
author_sort	Martínez,Claudio
title	Production of Rhodotorula glutinis: a yeast that secretes alpha-L-arabinofuranosidase
title_short	Production of Rhodotorula glutinis: a yeast that secretes alpha-L-arabinofuranosidase
title_full	Production of Rhodotorula glutinis: a yeast that secretes alpha-L-arabinofuranosidase
title_fullStr	Production of Rhodotorula glutinis: a yeast that secretes alpha-L-arabinofuranosidase
title_full_unstemmed	Production of Rhodotorula glutinis: a yeast that secretes alpha-L-arabinofuranosidase
title_sort	production of rhodotorula glutinis: a yeast that secretes alpha-l-arabinofuranosidase
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2006
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000400009
work_keys_str_mv	AT martinezclaudio productionofrhodotorulaglutinisayeastthatsecretesalphalarabinofuranosidase AT gertosiocecilia productionofrhodotorulaglutinisayeastthatsecretesalphalarabinofuranosidase AT labbeangelica productionofrhodotorulaglutinisayeastthatsecretesalphalarabinofuranosidase AT perezrafael productionofrhodotorulaglutinisayeastthatsecretesalphalarabinofuranosidase AT gangamariaangelica productionofrhodotorulaglutinisayeastthatsecretesalphalarabinofuranosidase
_version_	1718441754712080384

Production of Rhodotorula glutinis: a yeast that secretes alpha-L-arabinofuranosidase

Ejemplares similares