Deletion of DNA sequences of using a polymerase chain reaction based approach
We developed a simple, rapid and reliable method to delete DNA fragments in plasmids using a polymerase chain reaction based amplification of the circular DNA sequence that excludes the fragment to be deleted. The primers are designed to contain a non-complementary 5' sequence consisting of a r...
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| Auteurs principaux: | , , |
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| Langue: | English |
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Pontificia Universidad Católica de Valparaíso
2006
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| Accès en ligne: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500018 |
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