Deletion of DNA sequences of using a polymerase chain reaction based approach

Deletion of DNA sequences of using a polymerase chain reaction based approach

We developed a simple, rapid and reliable method to delete DNA fragments in plasmids using a polymerase chain reaction based amplification of the circular DNA sequence that excludes the fragment to be deleted. The primers are designed to contain a non-complementary 5' sequence consisting of a r...

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Autores principales: Pérez-Pinera,Pablo, Menéndez-González,Manuel, Vega,José Antonio
Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso 2006
Materias:
deletion
mutagenesis
polymerase chain reaction
plasmid
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000500018
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