Ethanol mediated enhancement in bacterial transformation

In molecular biology, transformation using E. coli as a host plays a key role in synthesizing gene libraries. The present study demonstrated a new ethanol-based method for transformation of plasmid DNA to E. coli. Ethanol at 10% concentration (v/v) showed best results. Further, as compared with trad...

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Autores principales: Sharma,Arun Dev, Singh,Jaspreet, Gill,Prabhjot Kaur
Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso 2007
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100016
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