Ethanol mediated enhancement in bacterial transformation

In molecular biology, transformation using E. coli as a host plays a key role in synthesizing gene libraries. The present study demonstrated a new ethanol-based method for transformation of plasmid DNA to E. coli. Ethanol at 10% concentration (v/v) showed best results. Further, as compared with trad...

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Autores principales:	Sharma,Arun Dev, Singh,Jaspreet, Gill,Prabhjot Kaur
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2007
Materias:	bacteria ethanol transformation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100016
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spelling	oai:scielo:S0717-345820070001000162007-08-14Ethanol mediated enhancement in bacterial transformationSharma,Arun DevSingh,JaspreetGill,Prabhjot Kaur bacteria ethanol transformation In molecular biology, transformation using E. coli as a host plays a key role in synthesizing gene libraries. The present study demonstrated a new ethanol-based method for transformation of plasmid DNA to E. coli. Ethanol at 10% concentration (v/v) showed best results. Further, as compared with traditional CaCl2 method, the transformation rate, using protocol outlined in this study, was very high, suggesting amenable for further applications.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.10 n.1 20072007-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100016en10.4067/S0717-34582007000100016
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	bacteria ethanol transformation
spellingShingle	bacteria ethanol transformation Sharma,Arun Dev Singh,Jaspreet Gill,Prabhjot Kaur Ethanol mediated enhancement in bacterial transformation
description	In molecular biology, transformation using E. coli as a host plays a key role in synthesizing gene libraries. The present study demonstrated a new ethanol-based method for transformation of plasmid DNA to E. coli. Ethanol at 10% concentration (v/v) showed best results. Further, as compared with traditional CaCl2 method, the transformation rate, using protocol outlined in this study, was very high, suggesting amenable for further applications.
author	Sharma,Arun Dev Singh,Jaspreet Gill,Prabhjot Kaur
author_facet	Sharma,Arun Dev Singh,Jaspreet Gill,Prabhjot Kaur
author_sort	Sharma,Arun Dev
title	Ethanol mediated enhancement in bacterial transformation
title_short	Ethanol mediated enhancement in bacterial transformation
title_full	Ethanol mediated enhancement in bacterial transformation
title_fullStr	Ethanol mediated enhancement in bacterial transformation
title_full_unstemmed	Ethanol mediated enhancement in bacterial transformation
title_sort	ethanol mediated enhancement in bacterial transformation
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2007
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100016
work_keys_str_mv	AT sharmaarundev ethanolmediatedenhancementinbacterialtransformation AT singhjaspreet ethanolmediatedenhancementinbacterialtransformation AT gillprabhjotkaur ethanolmediatedenhancementinbacterialtransformation
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Ethanol mediated enhancement in bacterial transformation

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