Ethanol mediated enhancement in bacterial transformation
In molecular biology, transformation using E. coli as a host plays a key role in synthesizing gene libraries. The present study demonstrated a new ethanol-based method for transformation of plasmid DNA to E. coli. Ethanol at 10% concentration (v/v) showed best results. Further, as compared with trad...
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Autores principales: | , , |
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Lenguaje: | English |
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Pontificia Universidad Católica de Valparaíso
2007
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Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100016 |
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