Multiple pulses improve electroporation efficiency in Agrobacterium tumefaciens
Electroporation entails brief, high intensity pulse to create transient pores in the cell membrane to facilitate the entry of exogenous macromolecules, which may otherwise be excluded. Removal of the external field leads to the resealing of the membrane electropores permitting the survival of the el...
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Langue: | English |
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Pontificia Universidad Católica de Valparaíso
2008
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Accès en ligne: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000100015 |
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