A phage display combined with DNA affinity magnetic system can be applied to a screening of DNA binding proteins, such as transcription factors

Here we introduce a new approach for the screening of DNA binding proteins, using a phage library based on a phage display technique. In principal, a complementary DNA (cDNA) library based on the recombinant bacteriophage T7 expressing target proteins on its capsid (phage display) is constructed. Th...

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Autores principales: Yulita,Kusumadewi Sri, Kouno,Takafumi, Ezaki,Bunichi
Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso 2010
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100014
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