Characterization of the antiyeast compound and probiotic properties of a starter Lactobacillus plantarum DW3 for possible use in fermented plant beverages

Lactobacillus plantarum DW3 produced antifungal compounds that inhibited the growth of Rhodotorula mucilaginosa DKA, contaminating yeast in fermented plant beverages (FPBs) and various potential human pathogens. Phenyllactic acid (PLA) identified by gas chromatography- mass spectrometry (GC-MS) was...

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Autores principales:	Kantachote,Duangporn, Prachyakij,Pakorn, Charernjiratrakul,Wilawan, Ongsakul,Metta, Duangjitcharoen,Yodsawee, Chaiyasut,Chaiyavat, Nitoda,Teruhiko, Kanzaki,Hiroshi
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2010
Materias:	fermented plant beverage Lactobacillus plantarum phenyllactic acid probiotics Rhodotorula mucilaginosa weight control
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500002
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spelling	oai:scielo:S0717-345820100005000022011-05-24Characterization of the antiyeast compound and probiotic properties of a starter Lactobacillus plantarum DW3 for possible use in fermented plant beveragesKantachote,DuangpornPrachyakij,PakornCharernjiratrakul,WilawanOngsakul,MettaDuangjitcharoen,YodsaweeChaiyasut,ChaiyavatNitoda,TeruhikoKanzaki,Hiroshi fermented plant beverage Lactobacillus plantarum phenyllactic acid probiotics Rhodotorula mucilaginosa weight control Lactobacillus plantarum DW3 produced antifungal compounds that inhibited the growth of Rhodotorula mucilaginosa DKA, contaminating yeast in fermented plant beverages (FPBs) and various potential human pathogens. Phenyllactic acid (PLA) identified by gas chromatography- mass spectrometry (GC-MS) was produced at 31 mg/L PLA in MRS medium and 5 mg/ml inhibited growth of the target yeast in vitro by 90%. Other inhibitors were also present but not specifically identified. Results of in vitro tests showed that DW3 also had probiotic properties as it survived various human biological barriers resistance to pH 3, bile salts, growth without vitamin B12 and the presence and absence of oxygen. Its inhibitory effect against food borne pathogenic bacteria and spoilage organisms was higher than that found for a commercial strain Lactobacillus casei R. An acute oral toxicity test on ICR mice at a high single dose of either 10(9) and 10(12) cells per mouse for 14 days showed that DW3 had no adverse effect on the general health status and there was no evidence of bacteremia. Mice fed DW3 had a reduced weight gain compared to the control. No significant difference (p > 0.05) was found for the spleen weight index (SWI) among the treatment and control groups whereas there was a significant difference (p < 0.05) for the liver weight ratio (LWR) in a group fed with 10(12) cells per mouse when compared with the control group.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.13 n.5 20102010-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500002en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	fermented plant beverage Lactobacillus plantarum phenyllactic acid probiotics Rhodotorula mucilaginosa weight control
spellingShingle	fermented plant beverage Lactobacillus plantarum phenyllactic acid probiotics Rhodotorula mucilaginosa weight control Kantachote,Duangporn Prachyakij,Pakorn Charernjiratrakul,Wilawan Ongsakul,Metta Duangjitcharoen,Yodsawee Chaiyasut,Chaiyavat Nitoda,Teruhiko Kanzaki,Hiroshi Characterization of the antiyeast compound and probiotic properties of a starter Lactobacillus plantarum DW3 for possible use in fermented plant beverages
description	Lactobacillus plantarum DW3 produced antifungal compounds that inhibited the growth of Rhodotorula mucilaginosa DKA, contaminating yeast in fermented plant beverages (FPBs) and various potential human pathogens. Phenyllactic acid (PLA) identified by gas chromatography- mass spectrometry (GC-MS) was produced at 31 mg/L PLA in MRS medium and 5 mg/ml inhibited growth of the target yeast in vitro by 90%. Other inhibitors were also present but not specifically identified. Results of in vitro tests showed that DW3 also had probiotic properties as it survived various human biological barriers resistance to pH 3, bile salts, growth without vitamin B12 and the presence and absence of oxygen. Its inhibitory effect against food borne pathogenic bacteria and spoilage organisms was higher than that found for a commercial strain Lactobacillus casei R. An acute oral toxicity test on ICR mice at a high single dose of either 10(9) and 10(12) cells per mouse for 14 days showed that DW3 had no adverse effect on the general health status and there was no evidence of bacteremia. Mice fed DW3 had a reduced weight gain compared to the control. No significant difference (p > 0.05) was found for the spleen weight index (SWI) among the treatment and control groups whereas there was a significant difference (p < 0.05) for the liver weight ratio (LWR) in a group fed with 10(12) cells per mouse when compared with the control group.
author	Kantachote,Duangporn Prachyakij,Pakorn Charernjiratrakul,Wilawan Ongsakul,Metta Duangjitcharoen,Yodsawee Chaiyasut,Chaiyavat Nitoda,Teruhiko Kanzaki,Hiroshi
author_facet	Kantachote,Duangporn Prachyakij,Pakorn Charernjiratrakul,Wilawan Ongsakul,Metta Duangjitcharoen,Yodsawee Chaiyasut,Chaiyavat Nitoda,Teruhiko Kanzaki,Hiroshi
author_sort	Kantachote,Duangporn
title	Characterization of the antiyeast compound and probiotic properties of a starter Lactobacillus plantarum DW3 for possible use in fermented plant beverages
title_short	Characterization of the antiyeast compound and probiotic properties of a starter Lactobacillus plantarum DW3 for possible use in fermented plant beverages
title_full	Characterization of the antiyeast compound and probiotic properties of a starter Lactobacillus plantarum DW3 for possible use in fermented plant beverages
title_fullStr	Characterization of the antiyeast compound and probiotic properties of a starter Lactobacillus plantarum DW3 for possible use in fermented plant beverages
title_full_unstemmed	Characterization of the antiyeast compound and probiotic properties of a starter Lactobacillus plantarum DW3 for possible use in fermented plant beverages
title_sort	characterization of the antiyeast compound and probiotic properties of a starter lactobacillus plantarum dw3 for possible use in fermented plant beverages
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2010
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500002
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Characterization of the antiyeast compound and probiotic properties of a starter Lactobacillus plantarum DW3 for possible use in fermented plant beverages

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