Optimization of polyethylenimine-mediated transient transfection using response surface methodology design
Response surface methodology was undertaken to optimize the polyethylenimine-mediated transient transfection of suspension cultured HEK 293-F cells. A total of 15 combinations were designed according to Box-Behnken design to identify the effects of DNA concentration, polyethylenimine concentration a...
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| Auteurs principaux: | , |
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| Langue: | English |
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Pontificia Universidad Católica de Valparaíso
2010
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| Accès en ligne: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500010 |
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