Isolation of total RNA from hard bamboo tissue rich in polyphenols and polysaccharides for gene expression studies

RNA isolation from hard and woody internodal bamboo (Bambusa balcooa) tissue is very difficult due to the presence of secondary metabolites, polysaccharides, and polyphenolics. These compounds often co-precipitate with isolated RNA and hinder downstream applications. We have developed an efficient,...

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Auteurs principaux: Rai,Vineeta, Ghosh,Jayadri Sekhar, Dey,Nrisingha
Langue:English
Publié: Pontificia Universidad Católica de Valparaíso 2010
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Accès en ligne:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500022
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