Isolation of total RNA from hard bamboo tissue rich in polyphenols and polysaccharides for gene expression studies
RNA isolation from hard and woody internodal bamboo (Bambusa balcooa) tissue is very difficult due to the presence of secondary metabolites, polysaccharides, and polyphenolics. These compounds often co-precipitate with isolated RNA and hinder downstream applications. We have developed an efficient,...
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Autores principales: | Rai,Vineeta, Ghosh,Jayadri Sekhar, Dey,Nrisingha |
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Lenguaje: | English |
Publicado: |
Pontificia Universidad Católica de Valparaíso
2010
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Materias: | |
Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000500022 |
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