A simple ethanol wash of the tissue homogenates recovers high-quality genomic DNA from Corchorus species characterized by highly acidic and proteinaceous mucilages

A simple miniprep based on early elimination of highly acidic and proteinaceous mucilages through ethanol washing of the tissue homogenates has been developed for the extraction of genomic DNA from mature leaves and seeds of Corchorus spp. As compared to high cetyltrimethylammonium bromide (CTAB)-Na...

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Autores principales:	Kundu,Avijit, Sarkar,Debabrata, Bhattacharjee,Amit, Topdar,Niladri, Sinha,Mohit Kumar, Mahapatra,Bikash Sinha
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2011
Materias:	Corchorus DNA extraction microsatellite mucilage PCR restriction digestion
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100010
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spelling	oai:scielo:S0717-345820110001000102011-05-24A simple ethanol wash of the tissue homogenates recovers high-quality genomic DNA from Corchorus species characterized by highly acidic and proteinaceous mucilagesKundu,AvijitSarkar,DebabrataBhattacharjee,AmitTopdar,NiladriSinha,Mohit KumarMahapatra,Bikash Sinha Corchorus DNA extraction microsatellite mucilage PCR restriction digestion A simple miniprep based on early elimination of highly acidic and proteinaceous mucilages through ethanol washing of the tissue homogenates has been developed for the extraction of genomic DNA from mature leaves and seeds of Corchorus spp. As compared to high cetyltrimethylammonium bromide (CTAB)-NaCl DNA extraction followed by ethanol-based removal of remnant mucilages from the DNA pellet, this simple miniprep consistently and reproducibly recovers high amounts of DNA with good spectral qualities at A260/A280 and A260/A230. The purified DNA is efficiently digested by restriction endonucleases, and is suitable for PCR amplification of nuclear microsatellites with expected allele sizes.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.14 n.1 20112011-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100010en
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Corchorus DNA extraction microsatellite mucilage PCR restriction digestion
spellingShingle	Corchorus DNA extraction microsatellite mucilage PCR restriction digestion Kundu,Avijit Sarkar,Debabrata Bhattacharjee,Amit Topdar,Niladri Sinha,Mohit Kumar Mahapatra,Bikash Sinha A simple ethanol wash of the tissue homogenates recovers high-quality genomic DNA from Corchorus species characterized by highly acidic and proteinaceous mucilages
description	A simple miniprep based on early elimination of highly acidic and proteinaceous mucilages through ethanol washing of the tissue homogenates has been developed for the extraction of genomic DNA from mature leaves and seeds of Corchorus spp. As compared to high cetyltrimethylammonium bromide (CTAB)-NaCl DNA extraction followed by ethanol-based removal of remnant mucilages from the DNA pellet, this simple miniprep consistently and reproducibly recovers high amounts of DNA with good spectral qualities at A260/A280 and A260/A230. The purified DNA is efficiently digested by restriction endonucleases, and is suitable for PCR amplification of nuclear microsatellites with expected allele sizes.
author	Kundu,Avijit Sarkar,Debabrata Bhattacharjee,Amit Topdar,Niladri Sinha,Mohit Kumar Mahapatra,Bikash Sinha
author_facet	Kundu,Avijit Sarkar,Debabrata Bhattacharjee,Amit Topdar,Niladri Sinha,Mohit Kumar Mahapatra,Bikash Sinha
author_sort	Kundu,Avijit
title	A simple ethanol wash of the tissue homogenates recovers high-quality genomic DNA from Corchorus species characterized by highly acidic and proteinaceous mucilages
title_short	A simple ethanol wash of the tissue homogenates recovers high-quality genomic DNA from Corchorus species characterized by highly acidic and proteinaceous mucilages
title_full	A simple ethanol wash of the tissue homogenates recovers high-quality genomic DNA from Corchorus species characterized by highly acidic and proteinaceous mucilages
title_fullStr	A simple ethanol wash of the tissue homogenates recovers high-quality genomic DNA from Corchorus species characterized by highly acidic and proteinaceous mucilages
title_full_unstemmed	A simple ethanol wash of the tissue homogenates recovers high-quality genomic DNA from Corchorus species characterized by highly acidic and proteinaceous mucilages
title_sort	simple ethanol wash of the tissue homogenates recovers high-quality genomic dna from corchorus species characterized by highly acidic and proteinaceous mucilages
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2011
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100010
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A simple ethanol wash of the tissue homogenates recovers high-quality genomic DNA from Corchorus species characterized by highly acidic and proteinaceous mucilages

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