A simple ethanol wash of the tissue homogenates recovers high-quality genomic DNA from Corchorus species characterized by highly acidic and proteinaceous mucilages

A simple miniprep based on early elimination of highly acidic and proteinaceous mucilages through ethanol washing of the tissue homogenates has been developed for the extraction of genomic DNA from mature leaves and seeds of Corchorus spp. As compared to high cetyltrimethylammonium bromide (CTAB)-Na...

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Autores principales: Kundu,Avijit, Sarkar,Debabrata, Bhattacharjee,Amit, Topdar,Niladri, Sinha,Mohit Kumar, Mahapatra,Bikash Sinha
Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso 2011
Materias:
PCR
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100010
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