A simple ethanol wash of the tissue homogenates recovers high-quality genomic DNA from Corchorus species characterized by highly acidic and proteinaceous mucilages

A simple ethanol wash of the tissue homogenates recovers high-quality genomic DNA from Corchorus species characterized by highly acidic and proteinaceous mucilages

A simple miniprep based on early elimination of highly acidic and proteinaceous mucilages through ethanol washing of the tissue homogenates has been developed for the extraction of genomic DNA from mature leaves and seeds of Corchorus spp. As compared to high cetyltrimethylammonium bromide (CTAB)-Na...

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Bibliographic Details
Main Authors: Kundu,Avijit, Sarkar,Debabrata, Bhattacharjee,Amit, Topdar,Niladri, Sinha,Mohit Kumar, Mahapatra,Bikash Sinha
Language:English
Published: Pontificia Universidad Católica de Valparaíso 2011
Subjects:
Corchorus
DNA extraction
microsatellite
mucilage
PCR
restriction digestion
Online Access:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100010
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