Codon optimization of 1,3-propanediol oxidoreductase expression in Escherichia coli and enzymatic properties

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Codon optimization of 1,3-propanediol oxidoreductase expression in Escherichia coli and enzymatic properties

The gene dhaT from Klebsiella pneumoniae encoding 1,3-propanediol oxidoreductase (PDOR) was de novo synthesized by splicing overlap extension polymerase chain reaction (SOE-PCR) primarily according to Escherichia colis codon usage, as well as mRNA secondary structure. After optimization, Codon Adap...

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Auteurs principaux:	Li,Wei, Ng,I-Son, Fang,Baishan, Yu,Jincong, Zhang,Guangya
Langue:	English
Publié:	Pontificia Universidad Católica de Valparaíso 2011
Sujets:	1,3-propanediol oxidoreductase codon optimization enzymatic properties Escherichia coli overlap extension PCR
Accès en ligne:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000400007
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