Codon optimization of 1,3-propanediol oxidoreductase expression in Escherichia coli and enzymatic properties
The gene dhaT from Klebsiella pneumoniae encoding 1,3-propanediol oxidoreductase (PDOR) was de novo synthesized by splicing overlap extension polymerase chain reaction (SOE-PCR) primarily according to Escherichia colis codon usage, as well as mRNA secondary structure. After optimization, Codon Adap...
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| Main Authors: | , , , , |
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| Language: | English |
| Published: |
Pontificia Universidad Católica de Valparaíso
2011
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| Subjects: | |
| Online Access: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000400007 |
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