Aspergillus kawachii produces an inulinase in cultures with yacon (Smallanthus sonchifolius) as substrate

Background: Inulinases have been extracted and characterized from inulin-storing tissues; however, production of microbial inulinases have recently draw much attention as they offer several industrial advantages. Many microorganisms, including filamentous fungi, yeast and bacteria have been claimed...

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Autores principales: Chesini,Mariana, Neila,Lorena Paola, de la Parra,Dante Fratebianchi, Rojas,Natalia Lorena, Contreras Esquivel,Juan Carlos, Cavalitto,Sebastian Fernando, Ghiringhelli,Pablo Daniel, Hours,Roque Alberto
Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso 2013
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Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000300008
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spelling oai:scielo:S0717-345820130003000082013-08-22Aspergillus kawachii produces an inulinase in cultures with yacon (Smallanthus sonchifolius) as substrateChesini,MarianaNeila,Lorena Paolade la Parra,Dante FratebianchiRojas,Natalia LorenaContreras Esquivel,Juan CarlosCavalitto,Sebastian FernandoGhiringhelli,Pablo DanielHours,Roque Alberto acidophilic enzymes batch cultures inulin degradation submerged cultures Background: Inulinases have been extracted and characterized from inulin-storing tissues; however, production of microbial inulinases have recently draw much attention as they offer several industrial advantages. Many microorganisms, including filamentous fungi, yeast and bacteria have been claimed as inulinase producers. These hydrolases are usually inducible and their exo-acting forms may hydrolyze fructose polymers (inulin) and oligosaccharides such as sucrose and raffinose. Fungal inulinase extracts are often produced as stable mixture of highly active fructanhydrolases. From a practical prospective, the best known inulinases to date are those produced by species of Penicillium, Aspergillus and Kluyveromyces. Results: The production of extracellular inulinase by A. kawachii in liquid cultures, using either inulin or yacon derived materials as CES as well as inulinase inducers, is reported. In addition, a partial characterization of the enzyme activity is included. Conclusions: Yacon derived products, particularly yacon juice, added to the culture medium proved to be a good CES for fungal growth as well as an inducer of enzyme synthesis. Partial characterization of the enzyme revealed that it is quite stable in a wide range of pH and temperature. In addition, characterization of the reaction products revealed that this enzyme corresponds to an exo-type. These facts are promising considering its potential application in inulin hydrolysis for the production of high fructose syrups.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.16 n.3 20132013-05-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000300008en10.2225/vol16-issue3-fulltext-13
institution Scielo Chile
collection Scielo Chile
language English
topic acidophilic enzymes
batch cultures
inulin degradation
submerged cultures
spellingShingle acidophilic enzymes
batch cultures
inulin degradation
submerged cultures
Chesini,Mariana
Neila,Lorena Paola
de la Parra,Dante Fratebianchi
Rojas,Natalia Lorena
Contreras Esquivel,Juan Carlos
Cavalitto,Sebastian Fernando
Ghiringhelli,Pablo Daniel
Hours,Roque Alberto
Aspergillus kawachii produces an inulinase in cultures with yacon (Smallanthus sonchifolius) as substrate
description Background: Inulinases have been extracted and characterized from inulin-storing tissues; however, production of microbial inulinases have recently draw much attention as they offer several industrial advantages. Many microorganisms, including filamentous fungi, yeast and bacteria have been claimed as inulinase producers. These hydrolases are usually inducible and their exo-acting forms may hydrolyze fructose polymers (inulin) and oligosaccharides such as sucrose and raffinose. Fungal inulinase extracts are often produced as stable mixture of highly active fructanhydrolases. From a practical prospective, the best known inulinases to date are those produced by species of Penicillium, Aspergillus and Kluyveromyces. Results: The production of extracellular inulinase by A. kawachii in liquid cultures, using either inulin or yacon derived materials as CES as well as inulinase inducers, is reported. In addition, a partial characterization of the enzyme activity is included. Conclusions: Yacon derived products, particularly yacon juice, added to the culture medium proved to be a good CES for fungal growth as well as an inducer of enzyme synthesis. Partial characterization of the enzyme revealed that it is quite stable in a wide range of pH and temperature. In addition, characterization of the reaction products revealed that this enzyme corresponds to an exo-type. These facts are promising considering its potential application in inulin hydrolysis for the production of high fructose syrups.
author Chesini,Mariana
Neila,Lorena Paola
de la Parra,Dante Fratebianchi
Rojas,Natalia Lorena
Contreras Esquivel,Juan Carlos
Cavalitto,Sebastian Fernando
Ghiringhelli,Pablo Daniel
Hours,Roque Alberto
author_facet Chesini,Mariana
Neila,Lorena Paola
de la Parra,Dante Fratebianchi
Rojas,Natalia Lorena
Contreras Esquivel,Juan Carlos
Cavalitto,Sebastian Fernando
Ghiringhelli,Pablo Daniel
Hours,Roque Alberto
author_sort Chesini,Mariana
title Aspergillus kawachii produces an inulinase in cultures with yacon (Smallanthus sonchifolius) as substrate
title_short Aspergillus kawachii produces an inulinase in cultures with yacon (Smallanthus sonchifolius) as substrate
title_full Aspergillus kawachii produces an inulinase in cultures with yacon (Smallanthus sonchifolius) as substrate
title_fullStr Aspergillus kawachii produces an inulinase in cultures with yacon (Smallanthus sonchifolius) as substrate
title_full_unstemmed Aspergillus kawachii produces an inulinase in cultures with yacon (Smallanthus sonchifolius) as substrate
title_sort aspergillus kawachii produces an inulinase in cultures with yacon (smallanthus sonchifolius) as substrate
publisher Pontificia Universidad Católica de Valparaíso
publishDate 2013
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000300008
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