The ICY1 gene from Saccharomyces cerevisiae affects nitrogen consumption during alcoholic fermentation

Background Saccharomyces cerevisiae is the main microorganism responsible for alcoholic fermentation. In this process, the consumption of nitrogen is of great importance since it is found in limiting quantities and its deficiency produces sluggish and/or stuck fermentations generating large economic...

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Autores principales:	Martínez,Claudio, Contreras,Angela, Aguilera,Omayra, Ganga,Angelica, García,Veronica
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2014
Materias:	Enological traits Gene expression Wine fermentation Wine yeast
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000400002
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spelling	oai:scielo:S0717-345820140004000022014-09-02The ICY1 gene from Saccharomyces cerevisiae affects nitrogen consumption during alcoholic fermentationMartínez,ClaudioContreras,AngelaAguilera,OmayraGanga,AngelicaGarcía,Veronica Enological traits Gene expression Wine fermentation Wine yeast Background Saccharomyces cerevisiae is the main microorganism responsible for alcoholic fermentation. In this process, the consumption of nitrogen is of great importance since it is found in limiting quantities and its deficiency produces sluggish and/or stuck fermentations generating large economic losses in the wine-making industry. In a previous work we compared the transcriptional profiles between genetically related strains with differences in nitrogen consumption, detecting genes with differential expression that could be associated to the differences in the levels of nitrogen consumed. One of the genes identified was ICY1. With the aim of confirming this observation, in the present work we evaluated the consumption of ammonium during the fermentation of strains that have deleted or overexpressed this gene. Results Our results confirm the effect of ICY1 on nitrogen uptake by evaluating its expression in wine yeasts during the first stages of fermentation under low (MS60) and normal (MS300) assimilable nitrogen. Our results show that the mRNA levels of ICY1 diminish when the amount of assimilable nitrogen is low. Furthermore, we constructed strains derived from the industrial strain EC1118 as a null mutant in this gene as well as one that overexpressed it. Conclusions Our results suggest that the expression of ICY1 is regulated by the amount of nitrogen available in the must and it is involved in the consumption of ammonium, given the increase in the consumption of this nitrogen source observed in the null mutant strain.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.17 n.4 20142014-07-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000400002en10.1016/j.ejbt.2014.04.006
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Enological traits Gene expression Wine fermentation Wine yeast
spellingShingle	Enological traits Gene expression Wine fermentation Wine yeast Martínez,Claudio Contreras,Angela Aguilera,Omayra Ganga,Angelica García,Veronica The ICY1 gene from Saccharomyces cerevisiae affects nitrogen consumption during alcoholic fermentation
description	Background Saccharomyces cerevisiae is the main microorganism responsible for alcoholic fermentation. In this process, the consumption of nitrogen is of great importance since it is found in limiting quantities and its deficiency produces sluggish and/or stuck fermentations generating large economic losses in the wine-making industry. In a previous work we compared the transcriptional profiles between genetically related strains with differences in nitrogen consumption, detecting genes with differential expression that could be associated to the differences in the levels of nitrogen consumed. One of the genes identified was ICY1. With the aim of confirming this observation, in the present work we evaluated the consumption of ammonium during the fermentation of strains that have deleted or overexpressed this gene. Results Our results confirm the effect of ICY1 on nitrogen uptake by evaluating its expression in wine yeasts during the first stages of fermentation under low (MS60) and normal (MS300) assimilable nitrogen. Our results show that the mRNA levels of ICY1 diminish when the amount of assimilable nitrogen is low. Furthermore, we constructed strains derived from the industrial strain EC1118 as a null mutant in this gene as well as one that overexpressed it. Conclusions Our results suggest that the expression of ICY1 is regulated by the amount of nitrogen available in the must and it is involved in the consumption of ammonium, given the increase in the consumption of this nitrogen source observed in the null mutant strain.
author	Martínez,Claudio Contreras,Angela Aguilera,Omayra Ganga,Angelica García,Veronica
author_facet	Martínez,Claudio Contreras,Angela Aguilera,Omayra Ganga,Angelica García,Veronica
author_sort	Martínez,Claudio
title	The ICY1 gene from Saccharomyces cerevisiae affects nitrogen consumption during alcoholic fermentation
title_short	The ICY1 gene from Saccharomyces cerevisiae affects nitrogen consumption during alcoholic fermentation
title_full	The ICY1 gene from Saccharomyces cerevisiae affects nitrogen consumption during alcoholic fermentation
title_fullStr	The ICY1 gene from Saccharomyces cerevisiae affects nitrogen consumption during alcoholic fermentation
title_full_unstemmed	The ICY1 gene from Saccharomyces cerevisiae affects nitrogen consumption during alcoholic fermentation
title_sort	icy1 gene from saccharomyces cerevisiae affects nitrogen consumption during alcoholic fermentation
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2014
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000400002
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The ICY1 gene from Saccharomyces cerevisiae affects nitrogen consumption during alcoholic fermentation

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