SSR genetic diversity assessment of popular pigeonpea varieties in Malawi reveals unique fingerprints

Background: Pigeonpea (Cajanus cajan (L.) Millsp.) is a drought tolerant legume of the Fabaceae family and the only cultivated species in the genus Cajanus. It is mainly cultivated in the semi-arid tropics of Asia and Oceania, Africa and America. In Malawi, it is grown as a source of food and income...

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Autores principales:	Njung'e,Vincent, Deshpande,Santosh, Siambi,Moses, Jones,Richard, Silim,Said, De Villiers,Santie
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso 2016
Materias:	Genetic variation Genotyping Microsatellites Seed purity
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300008
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spelling	oai:scielo:S0717-345820160003000082016-07-06SSR genetic diversity assessment of popular pigeonpea varieties in Malawi reveals unique fingerprintsNjung'e,VincentDeshpande,SantoshSiambi,MosesJones,RichardSilim,SaidDe Villiers,Santie Genetic variation Genotyping Microsatellites Seed purity Background: Pigeonpea (Cajanus cajan (L.) Millsp.) is a drought tolerant legume of the Fabaceae family and the only cultivated species in the genus Cajanus. It is mainly cultivated in the semi-arid tropics of Asia and Oceania, Africa and America. In Malawi, it is grown as a source of food and income and for soil improvement in intercropping systems. However, varietal contamination due to natural outcrossing causes significant quality reduction and yield losses. In this study, 48 polymorphic SSR markers were used to assess the diversity among all pigeonpea varieties cultivated in Malawi to determine if a genetic fingerprint could be identified to distinguish the popular varieties. Results: A total of 212 alleles were observed with an average of 5.58 alleles per marker and a maximum of 14 alleles produced by CCttc019 (Marker 40). Polymorphic information content (PIC), ranged from 0.03 to 0.89 with an average of 0.30. A neighbor-joining tree produced 4 clusters. The most commonly cultivated varieties, which include released varieties and cultivated land races, were well-spread across all the clusters observed, indicating that they generally represented the genetic diversity available in Malawi, although substantial variation was evident that can still be exploited through further breeding. Conclusion: Screening of the allelic data associated with the five most popular cultivated varieties, revealed 6 markers - CCB1, CCB7, Ccac035, CCttc003, Ccac026 and CCttc019 - which displayed unique allelic profiles for each of the five varieties. This genetic fingerprint can potentially be applied for seed certification to confirm the genetic purity of seeds that are delivered to Malawi farmers.info:eu-repo/semantics/openAccessPontificia Universidad Católica de ValparaísoElectronic Journal of Biotechnology v.19 n.3 20162016-05-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300008en10.1016/j.ejbt.2016.02.004
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Genetic variation Genotyping Microsatellites Seed purity
spellingShingle	Genetic variation Genotyping Microsatellites Seed purity Njung'e,Vincent Deshpande,Santosh Siambi,Moses Jones,Richard Silim,Said De Villiers,Santie SSR genetic diversity assessment of popular pigeonpea varieties in Malawi reveals unique fingerprints
description	Background: Pigeonpea (Cajanus cajan (L.) Millsp.) is a drought tolerant legume of the Fabaceae family and the only cultivated species in the genus Cajanus. It is mainly cultivated in the semi-arid tropics of Asia and Oceania, Africa and America. In Malawi, it is grown as a source of food and income and for soil improvement in intercropping systems. However, varietal contamination due to natural outcrossing causes significant quality reduction and yield losses. In this study, 48 polymorphic SSR markers were used to assess the diversity among all pigeonpea varieties cultivated in Malawi to determine if a genetic fingerprint could be identified to distinguish the popular varieties. Results: A total of 212 alleles were observed with an average of 5.58 alleles per marker and a maximum of 14 alleles produced by CCttc019 (Marker 40). Polymorphic information content (PIC), ranged from 0.03 to 0.89 with an average of 0.30. A neighbor-joining tree produced 4 clusters. The most commonly cultivated varieties, which include released varieties and cultivated land races, were well-spread across all the clusters observed, indicating that they generally represented the genetic diversity available in Malawi, although substantial variation was evident that can still be exploited through further breeding. Conclusion: Screening of the allelic data associated with the five most popular cultivated varieties, revealed 6 markers - CCB1, CCB7, Ccac035, CCttc003, Ccac026 and CCttc019 - which displayed unique allelic profiles for each of the five varieties. This genetic fingerprint can potentially be applied for seed certification to confirm the genetic purity of seeds that are delivered to Malawi farmers.
author	Njung'e,Vincent Deshpande,Santosh Siambi,Moses Jones,Richard Silim,Said De Villiers,Santie
author_facet	Njung'e,Vincent Deshpande,Santosh Siambi,Moses Jones,Richard Silim,Said De Villiers,Santie
author_sort	Njung'e,Vincent
title	SSR genetic diversity assessment of popular pigeonpea varieties in Malawi reveals unique fingerprints
title_short	SSR genetic diversity assessment of popular pigeonpea varieties in Malawi reveals unique fingerprints
title_full	SSR genetic diversity assessment of popular pigeonpea varieties in Malawi reveals unique fingerprints
title_fullStr	SSR genetic diversity assessment of popular pigeonpea varieties in Malawi reveals unique fingerprints
title_full_unstemmed	SSR genetic diversity assessment of popular pigeonpea varieties in Malawi reveals unique fingerprints
title_sort	ssr genetic diversity assessment of popular pigeonpea varieties in malawi reveals unique fingerprints
publisher	Pontificia Universidad Católica de Valparaíso
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300008
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SSR genetic diversity assessment of popular pigeonpea varieties in Malawi reveals unique fingerprints

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