Efficient expression and characterization of a cold-active endo-1, 4-β-glucanase from Citrobacter farmeri by co-expression of Myxococcus xanthus protein S

Efficient expression and characterization of a cold-active endo-1, 4-β-glucanase from Citrobacter farmeri by co-expression of Myxococcus xanthus protein S

Background: Cold-active endo-1, 4-β-glucanase (EglC) can decrease energy costs and prevent product denaturation in biotechnological processes. However, the nature EglC from C. farmeri A1 showed very low activity (800 U/L). In an attempt to increase its expression level, C. farmeri EglC was...

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Autores principales: Bai,Xi, Yuan,Xianjun, Wen,Aiyou, Li,Junfeng, Bai,Yunfeng, Shao,Tao
Lenguaje:English
Publicado: Pontificia Universidad Católica de Valparaíso 2016
Materias:
Cellulose degradation
Cellulose
Cold-active enzyme
Endoglucanases
Enzymatic properties
Escherichia coli
Expression
Novel expression vector
N-terminal fusion
Protein S-tag
Recombinant protein
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000600012
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http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000600012

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