Detection of Toxoplasma gondii in swine sausages

In order to evaluate the importance of swine sausages in toxoplasmosis epidemiology, Toxoplasma gondii presence was investigated in 70 samples of the product commercialized in the city of Botucatu-SP. Samples were analyzed by bioassay in mice and DNA amplification by Polymerase Chain Reaction (PCR)....

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Autores principales: DE OLIVEIRA MENDONÇA,ANDRÉ, DOMINGUES,PAULO F., VIEIRA DA SILVA,ARISTEU, BERGAMASCHI PEZERICO,SANDIA, LANGONI,HELIO
Lenguaje:English
Publicado: Sociedad Chilena de Parasitología 2004
Materias:
PCR
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-77122004000100008
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spelling oai:scielo:S0717-771220040001000082004-07-28Detection of Toxoplasma gondii in swine sausagesDE OLIVEIRA MENDONÇA,ANDRÉDOMINGUES,PAULO F.VIEIRA DA SILVA,ARISTEUBERGAMASCHI PEZERICO,SANDIALANGONI,HELIO Toxoplasma swine sausage PCR isolation In order to evaluate the importance of swine sausages in toxoplasmosis epidemiology, Toxoplasma gondii presence was investigated in 70 samples of the product commercialized in the city of Botucatu-SP. Samples were analyzed by bioassay in mice and DNA amplification by Polymerase Chain Reaction (PCR). Although the parasite was not isolated from any sample in the bioassay, 33 (47.14%) samples were positive in the PCR. These results indicate that swine sausages probably have low importance as a source of infection for human toxoplasmosis in the studied region. Nevertheless, the great number of PCR positive samples shows that the protozoan may be present, but may be inactivated by salt added in sausage manufactureinfo:eu-repo/semantics/openAccessSociedad Chilena de ParasitologíaParasitología latinoamericana v.59 n.1-2 20042004-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-77122004000100008en10.4067/S0717-77122004000100008
institution Scielo Chile
collection Scielo Chile
language English
topic Toxoplasma
swine sausage
PCR
isolation
spellingShingle Toxoplasma
swine sausage
PCR
isolation
DE OLIVEIRA MENDONÇA,ANDRÉ
DOMINGUES,PAULO F.
VIEIRA DA SILVA,ARISTEU
BERGAMASCHI PEZERICO,SANDIA
LANGONI,HELIO
Detection of Toxoplasma gondii in swine sausages
description In order to evaluate the importance of swine sausages in toxoplasmosis epidemiology, Toxoplasma gondii presence was investigated in 70 samples of the product commercialized in the city of Botucatu-SP. Samples were analyzed by bioassay in mice and DNA amplification by Polymerase Chain Reaction (PCR). Although the parasite was not isolated from any sample in the bioassay, 33 (47.14%) samples were positive in the PCR. These results indicate that swine sausages probably have low importance as a source of infection for human toxoplasmosis in the studied region. Nevertheless, the great number of PCR positive samples shows that the protozoan may be present, but may be inactivated by salt added in sausage manufacture
author DE OLIVEIRA MENDONÇA,ANDRÉ
DOMINGUES,PAULO F.
VIEIRA DA SILVA,ARISTEU
BERGAMASCHI PEZERICO,SANDIA
LANGONI,HELIO
author_facet DE OLIVEIRA MENDONÇA,ANDRÉ
DOMINGUES,PAULO F.
VIEIRA DA SILVA,ARISTEU
BERGAMASCHI PEZERICO,SANDIA
LANGONI,HELIO
author_sort DE OLIVEIRA MENDONÇA,ANDRÉ
title Detection of Toxoplasma gondii in swine sausages
title_short Detection of Toxoplasma gondii in swine sausages
title_full Detection of Toxoplasma gondii in swine sausages
title_fullStr Detection of Toxoplasma gondii in swine sausages
title_full_unstemmed Detection of Toxoplasma gondii in swine sausages
title_sort detection of toxoplasma gondii in swine sausages
publisher Sociedad Chilena de Parasitología
publishDate 2004
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-77122004000100008
work_keys_str_mv AT deoliveiramendoncaandre detectionoftoxoplasmagondiiinswinesausages
AT dominguespaulof detectionoftoxoplasmagondiiinswinesausages
AT vieiradasilvaaristeu detectionoftoxoplasmagondiiinswinesausages
AT bergamaschipezericosandia detectionoftoxoplasmagondiiinswinesausages
AT langonihelio detectionoftoxoplasmagondiiinswinesausages
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