Early Administration of Peroxisomicine A1 (T-514 Extracted from K. parvifolia Seeds) Causes Necrosis of Implanted TC-1 Cells without Affecting Target Organs in a Murine Model

Peroxisomicine A1 (PA1), one of the toxins isolated from seeds of plants of the Karwinskia genus, whose targets organs are the liver, kidney, and lungs. There is a selective toxicity in vitro to cancer-cell lines derived from the lungs, liver, and colon, compared to normal cell lines. PA1 caused apo...

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Autores principales:	Soto-Domínguez,Adolfo, Piñeyro-López,Alfredo, Saucedo-Cárdenas,Odila, Ramírez-Durónd,Rosalba, Waksman de Torres,Noemí, Sepúlveda-Saavedra,Julio
Lenguaje:	English
Publicado:	Sociedad Chilena de Anatomía 2012
Materias:	Antineoplasic effect Necrosis Peroxisomicine A1 Selective toxicity TC-1 cells
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000100051
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spelling	oai:scielo:S0717-950220120001000512012-06-08Early Administration of Peroxisomicine A1 (T-514 Extracted from K. parvifolia Seeds) Causes Necrosis of Implanted TC-1 Cells without Affecting Target Organs in a Murine ModelSoto-Domínguez,AdolfoPiñeyro-López,AlfredoSaucedo-Cárdenas,OdilaRamírez-Durónd,RosalbaWaksman de Torres,NoemíSepúlveda-Saavedra,Julio Antineoplasic effect Necrosis Peroxisomicine A1 Selective toxicity TC-1 cells Peroxisomicine A1 (PA1), one of the toxins isolated from seeds of plants of the Karwinskia genus, whose targets organs are the liver, kidney, and lungs. There is a selective toxicity in vitro to cancer-cell lines derived from the lungs, liver, and colon, compared to normal cell lines. PA1 caused apoptosis in several cancer-cell lines in culture. In toxic doses to rodents, it causes extensive apoptosis in the liver, kidney, and lungs. In our study we were interested in evaluating, for the first time, the morphological effects of administration of PA1 to implanted TC-1 cells and in the target organs in vivo. The TC-1 cells were cultured and injected into the hind limb of C57BL-6 mice. The animals were divided into 3 groups; those treated with four doses of 1 mg/kg each of PA1, the untreated control, and the vehicle-control groups. All mice were killed 10 days after cell implantation. Samples were obtained from TC-1 cells at the implantation site and from the liver, kidney, and lungs. The samples were processed for examination under light and electron microscopy. In the PA1-treated group, the TC-1 cells had necrosis, whereas in the control groups the tumor cells were undamaged. The target organs did not show any lesions. We demonstrated for the first time that there is a selective toxic effect of PA1 on the TC-1 cells in vivo.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.30 n.1 20122012-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000100051en10.4067/S0717-95022012000100051
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Antineoplasic effect Necrosis Peroxisomicine A1 Selective toxicity TC-1 cells
spellingShingle	Antineoplasic effect Necrosis Peroxisomicine A1 Selective toxicity TC-1 cells Soto-Domínguez,Adolfo Piñeyro-López,Alfredo Saucedo-Cárdenas,Odila Ramírez-Durónd,Rosalba Waksman de Torres,Noemí Sepúlveda-Saavedra,Julio Early Administration of Peroxisomicine A1 (T-514 Extracted from K. parvifolia Seeds) Causes Necrosis of Implanted TC-1 Cells without Affecting Target Organs in a Murine Model
description	Peroxisomicine A1 (PA1), one of the toxins isolated from seeds of plants of the Karwinskia genus, whose targets organs are the liver, kidney, and lungs. There is a selective toxicity in vitro to cancer-cell lines derived from the lungs, liver, and colon, compared to normal cell lines. PA1 caused apoptosis in several cancer-cell lines in culture. In toxic doses to rodents, it causes extensive apoptosis in the liver, kidney, and lungs. In our study we were interested in evaluating, for the first time, the morphological effects of administration of PA1 to implanted TC-1 cells and in the target organs in vivo. The TC-1 cells were cultured and injected into the hind limb of C57BL-6 mice. The animals were divided into 3 groups; those treated with four doses of 1 mg/kg each of PA1, the untreated control, and the vehicle-control groups. All mice were killed 10 days after cell implantation. Samples were obtained from TC-1 cells at the implantation site and from the liver, kidney, and lungs. The samples were processed for examination under light and electron microscopy. In the PA1-treated group, the TC-1 cells had necrosis, whereas in the control groups the tumor cells were undamaged. The target organs did not show any lesions. We demonstrated for the first time that there is a selective toxic effect of PA1 on the TC-1 cells in vivo.
author	Soto-Domínguez,Adolfo Piñeyro-López,Alfredo Saucedo-Cárdenas,Odila Ramírez-Durónd,Rosalba Waksman de Torres,Noemí Sepúlveda-Saavedra,Julio
author_facet	Soto-Domínguez,Adolfo Piñeyro-López,Alfredo Saucedo-Cárdenas,Odila Ramírez-Durónd,Rosalba Waksman de Torres,Noemí Sepúlveda-Saavedra,Julio
author_sort	Soto-Domínguez,Adolfo
title	Early Administration of Peroxisomicine A1 (T-514 Extracted from K. parvifolia Seeds) Causes Necrosis of Implanted TC-1 Cells without Affecting Target Organs in a Murine Model
title_short	Early Administration of Peroxisomicine A1 (T-514 Extracted from K. parvifolia Seeds) Causes Necrosis of Implanted TC-1 Cells without Affecting Target Organs in a Murine Model
title_full	Early Administration of Peroxisomicine A1 (T-514 Extracted from K. parvifolia Seeds) Causes Necrosis of Implanted TC-1 Cells without Affecting Target Organs in a Murine Model
title_fullStr	Early Administration of Peroxisomicine A1 (T-514 Extracted from K. parvifolia Seeds) Causes Necrosis of Implanted TC-1 Cells without Affecting Target Organs in a Murine Model
title_full_unstemmed	Early Administration of Peroxisomicine A1 (T-514 Extracted from K. parvifolia Seeds) Causes Necrosis of Implanted TC-1 Cells without Affecting Target Organs in a Murine Model
title_sort	early administration of peroxisomicine a1 (t-514 extracted from k. parvifolia seeds) causes necrosis of implanted tc-1 cells without affecting target organs in a murine model
publisher	Sociedad Chilena de Anatomía
publishDate	2012
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000100051
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Early Administration of Peroxisomicine A1 (T-514 Extracted from K. parvifolia Seeds) Causes Necrosis of Implanted TC-1 Cells without Affecting Target Organs in a Murine Model

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