Morphometric Analysis and Pattern of Protein and Gene Expression of Apoptosis in Focal Cerebral Ischemia in Rats and the Neuroprotetive Action of Hypothermia and Ketoprofen
SUMMARY: This study aimed to investigate the morphometric and the pattern of protein and gene expression related to the extrinsic apoptotic pathway in experimental focal cerebral ischemia and the hole of neuroprotection with hypothermia and ketoprofen. For this analysis, 120 rats were randomly divid...
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| Lenguaje: | English |
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Sociedad Chilena de Anatomía
2020
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oai:scielo:S0717-950220200003005232020-04-07Morphometric Analysis and Pattern of Protein and Gene Expression of Apoptosis in Focal Cerebral Ischemia in Rats and the Neuroprotetive Action of Hypothermia and KetoprofenTirapelli,Daniela P. CCirino,Mucio Luiz de AssisCarvalho,Camila A. Melo deNovais,Paulo CezarFigueredo,Rafael ZimakPorsani,Lucas BarbosaGula,Isabella StracieriRodrigues,Andressa RomualdoSilva,Jairo Pinheiro daPereira,Adriana LisLizarte Neto,Fermino SanchesSchimming,Bruno CesarTazima,Maria de Fátima Galli SoritaFazan,Valéria de Paula SassoliCarlotti-Jr,Carlos GilbertoTirapelli,Luis FernandoColli,Benedicto Oscar Apoptosis Gene expression Caspase-3 Cerebral ischemia Neuroprotection Hypothermia Ketoprofen SUMMARY: This study aimed to investigate the morphometric and the pattern of protein and gene expression related to the extrinsic apoptotic pathway in experimental focal cerebral ischemia and the hole of neuroprotection with hypothermia and ketoprofen. For this analysis, 120 rats were randomly divided into 3 groups (20 animals each): control - no surgery (20 animals); sham - simulation of surgery (20 animals); ischemic - focal ischemia for 1 hour, without reperfusion (80 animals) and divided into four subgroups with 20 animals each: ischemic + intraischemic hypothermia; ischemic + previous intravenous ketoprofen, and ischemic + hypothermia and ketoprofen. The infarct volume was measured using morphometric analysis of infarct areas defined by triphenyl tetrazolium chloride and the patterns of expression of the apoptosis genes (Fas, c-Flip, caspase-8 and caspase-3) and the apoptosis protein caspase-3 were evaluated by quantitative real-time PCR and immunohistochemistry, respectively. Hypo expression of genes of extrinsic pathway of apoptosis was observed: Fas receptor, c-Flip and caspase-8 in the ischemics areas. Increases in the gene and protein caspase-3 in the ischemic areas were also observed, and these increases were reduced by hypothermia and ketoprofen, also noted in the morphometric study. The caspases-3 increase suggests that this gene plays an important role in apoptosis, probably culminating in cell death and that the neuroprotective effect of hypothermia and ketoprofen is involved.info:eu-repo/semantics/openAccessSociedad Chilena de AnatomíaInternational Journal of Morphology v.38 n.3 20202020-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000300523en10.4067/S0717-95022020000300523 |
| institution |
Scielo Chile |
| collection |
Scielo Chile |
| language |
English |
| topic |
Apoptosis Gene expression Caspase-3 Cerebral ischemia Neuroprotection Hypothermia Ketoprofen |
| spellingShingle |
Apoptosis Gene expression Caspase-3 Cerebral ischemia Neuroprotection Hypothermia Ketoprofen Tirapelli,Daniela P. C Cirino,Mucio Luiz de Assis Carvalho,Camila A. Melo de Novais,Paulo Cezar Figueredo,Rafael Zimak Porsani,Lucas Barbosa Gula,Isabella Stracieri Rodrigues,Andressa Romualdo Silva,Jairo Pinheiro da Pereira,Adriana Lis Lizarte Neto,Fermino Sanches Schimming,Bruno Cesar Tazima,Maria de Fátima Galli Sorita Fazan,Valéria de Paula Sassoli Carlotti-Jr,Carlos Gilberto Tirapelli,Luis Fernando Colli,Benedicto Oscar Morphometric Analysis and Pattern of Protein and Gene Expression of Apoptosis in Focal Cerebral Ischemia in Rats and the Neuroprotetive Action of Hypothermia and Ketoprofen |
| description |
SUMMARY: This study aimed to investigate the morphometric and the pattern of protein and gene expression related to the extrinsic apoptotic pathway in experimental focal cerebral ischemia and the hole of neuroprotection with hypothermia and ketoprofen. For this analysis, 120 rats were randomly divided into 3 groups (20 animals each): control - no surgery (20 animals); sham - simulation of surgery (20 animals); ischemic - focal ischemia for 1 hour, without reperfusion (80 animals) and divided into four subgroups with 20 animals each: ischemic + intraischemic hypothermia; ischemic + previous intravenous ketoprofen, and ischemic + hypothermia and ketoprofen. The infarct volume was measured using morphometric analysis of infarct areas defined by triphenyl tetrazolium chloride and the patterns of expression of the apoptosis genes (Fas, c-Flip, caspase-8 and caspase-3) and the apoptosis protein caspase-3 were evaluated by quantitative real-time PCR and immunohistochemistry, respectively. Hypo expression of genes of extrinsic pathway of apoptosis was observed: Fas receptor, c-Flip and caspase-8 in the ischemics areas. Increases in the gene and protein caspase-3 in the ischemic areas were also observed, and these increases were reduced by hypothermia and ketoprofen, also noted in the morphometric study. The caspases-3 increase suggests that this gene plays an important role in apoptosis, probably culminating in cell death and that the neuroprotective effect of hypothermia and ketoprofen is involved. |
| author |
Tirapelli,Daniela P. C Cirino,Mucio Luiz de Assis Carvalho,Camila A. Melo de Novais,Paulo Cezar Figueredo,Rafael Zimak Porsani,Lucas Barbosa Gula,Isabella Stracieri Rodrigues,Andressa Romualdo Silva,Jairo Pinheiro da Pereira,Adriana Lis Lizarte Neto,Fermino Sanches Schimming,Bruno Cesar Tazima,Maria de Fátima Galli Sorita Fazan,Valéria de Paula Sassoli Carlotti-Jr,Carlos Gilberto Tirapelli,Luis Fernando Colli,Benedicto Oscar |
| author_facet |
Tirapelli,Daniela P. C Cirino,Mucio Luiz de Assis Carvalho,Camila A. Melo de Novais,Paulo Cezar Figueredo,Rafael Zimak Porsani,Lucas Barbosa Gula,Isabella Stracieri Rodrigues,Andressa Romualdo Silva,Jairo Pinheiro da Pereira,Adriana Lis Lizarte Neto,Fermino Sanches Schimming,Bruno Cesar Tazima,Maria de Fátima Galli Sorita Fazan,Valéria de Paula Sassoli Carlotti-Jr,Carlos Gilberto Tirapelli,Luis Fernando Colli,Benedicto Oscar |
| author_sort |
Tirapelli,Daniela P. C |
| title |
Morphometric Analysis and Pattern of Protein and Gene Expression of Apoptosis in Focal Cerebral Ischemia in Rats and the Neuroprotetive Action of Hypothermia and Ketoprofen |
| title_short |
Morphometric Analysis and Pattern of Protein and Gene Expression of Apoptosis in Focal Cerebral Ischemia in Rats and the Neuroprotetive Action of Hypothermia and Ketoprofen |
| title_full |
Morphometric Analysis and Pattern of Protein and Gene Expression of Apoptosis in Focal Cerebral Ischemia in Rats and the Neuroprotetive Action of Hypothermia and Ketoprofen |
| title_fullStr |
Morphometric Analysis and Pattern of Protein and Gene Expression of Apoptosis in Focal Cerebral Ischemia in Rats and the Neuroprotetive Action of Hypothermia and Ketoprofen |
| title_full_unstemmed |
Morphometric Analysis and Pattern of Protein and Gene Expression of Apoptosis in Focal Cerebral Ischemia in Rats and the Neuroprotetive Action of Hypothermia and Ketoprofen |
| title_sort |
morphometric analysis and pattern of protein and gene expression of apoptosis in focal cerebral ischemia in rats and the neuroprotetive action of hypothermia and ketoprofen |
| publisher |
Sociedad Chilena de Anatomía |
| publishDate |
2020 |
| url |
http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022020000300523 |
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