KINETICS OF P - NITROPHENYL ACETATE HYDROLYSIS CATALYZED BY α- CHYMOTRYPSIN IN PRESENCE OF POLYETHYLENE GLYCOL

KINETICS OF P - NITROPHENYL ACETATE HYDROLYSIS CATALYZED BY α- CHYMOTRYPSIN IN PRESENCE OF POLYETHYLENE GLYCOL

The effect of poly (ethyleneglycol) (PEG) addition on the rate of p-nitrophenyl acetate (PNPA) catalyzed by a- chymotrypsin has been measured in TRIS buffer at pH = 7.4. The presence of PEG (up to 30 % p/v) leads to an increase in the rate of the process. The effect of PEG is evident both, in the pr...

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Autores principales: ABUIN,ELSA, LISSI,EDUARDO, BRIDI,RAQUEL
Lenguaje:English
Publicado: Sociedad Chilena de Química 2011
Acceso en línea:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072011000400028
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spelling oai:scielo:S0717-970720110004000282012-03-06KINETICS OF P - NITROPHENYL ACETATE HYDROLYSIS CATALYZED BY α- CHYMOTRYPSIN IN PRESENCE OF POLYETHYLENE GLYCOLABUIN,ELSALISSI,EDUARDOBRIDI,RAQUELThe effect of poly (ethyleneglycol) (PEG) addition on the rate of p-nitrophenyl acetate (PNPA) catalyzed by a- chymotrypsin has been measured in TRIS buffer at pH = 7.4. The presence of PEG (up to 30 % p/v) leads to an increase in the rate of the process. The effect of PEG is evident both, in the pre-steady state and steady state stages of the process. The reaction follows a Michaelis-Menten mechanism under all the conditions employed. The Michaelis constant slightly decreases and the catalytic rate constant slightly increases with PEG concentration over all the polymer concentration range employed (up to 30%). The decrease in the Michaelis constant suggests that the effect of the polymer is due to crowding.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.56 n.4 20112011-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072011000400028en10.4067/S0717-97072011000400028
institution Scielo Chile
collection Scielo Chile
language English
description The effect of poly (ethyleneglycol) (PEG) addition on the rate of p-nitrophenyl acetate (PNPA) catalyzed by a- chymotrypsin has been measured in TRIS buffer at pH = 7.4. The presence of PEG (up to 30 % p/v) leads to an increase in the rate of the process. The effect of PEG is evident both, in the pre-steady state and steady state stages of the process. The reaction follows a Michaelis-Menten mechanism under all the conditions employed. The Michaelis constant slightly decreases and the catalytic rate constant slightly increases with PEG concentration over all the polymer concentration range employed (up to 30%). The decrease in the Michaelis constant suggests that the effect of the polymer is due to crowding.
author ABUIN,ELSA
LISSI,EDUARDO
BRIDI,RAQUEL
spellingShingle ABUIN,ELSA
LISSI,EDUARDO
BRIDI,RAQUEL
KINETICS OF P - NITROPHENYL ACETATE HYDROLYSIS CATALYZED BY α- CHYMOTRYPSIN IN PRESENCE OF POLYETHYLENE GLYCOL
author_facet ABUIN,ELSA
LISSI,EDUARDO
BRIDI,RAQUEL
author_sort ABUIN,ELSA
title KINETICS OF P - NITROPHENYL ACETATE HYDROLYSIS CATALYZED BY α- CHYMOTRYPSIN IN PRESENCE OF POLYETHYLENE GLYCOL
title_short KINETICS OF P - NITROPHENYL ACETATE HYDROLYSIS CATALYZED BY α- CHYMOTRYPSIN IN PRESENCE OF POLYETHYLENE GLYCOL
title_full KINETICS OF P - NITROPHENYL ACETATE HYDROLYSIS CATALYZED BY α- CHYMOTRYPSIN IN PRESENCE OF POLYETHYLENE GLYCOL
title_fullStr KINETICS OF P - NITROPHENYL ACETATE HYDROLYSIS CATALYZED BY α- CHYMOTRYPSIN IN PRESENCE OF POLYETHYLENE GLYCOL
title_full_unstemmed KINETICS OF P - NITROPHENYL ACETATE HYDROLYSIS CATALYZED BY α- CHYMOTRYPSIN IN PRESENCE OF POLYETHYLENE GLYCOL
title_sort kinetics of p - nitrophenyl acetate hydrolysis catalyzed by α- chymotrypsin in presence of polyethylene glycol
publisher Sociedad Chilena de Química
publishDate 2011
url http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072011000400028
work_keys_str_mv AT abuinelsa kineticsofpnitrophenylacetatehydrolysiscatalyzedby945chymotrypsininpresenceofpolyethyleneglycol
AT lissieduardo kineticsofpnitrophenylacetatehydrolysiscatalyzedby945chymotrypsininpresenceofpolyethyleneglycol
AT bridiraquel kineticsofpnitrophenylacetatehydrolysiscatalyzedby945chymotrypsininpresenceofpolyethyleneglycol
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