ENANTIOMERIC LC SEPARATION OF METOLAZONE ON AMYLOSE BASED SORBENT

A simple, rapid and robust LC method was developed and validated for the enantiomeric separation of metolazone. The enantiomers of metolazone were resolved on a Chiralpak AD-H (amylose based stationary phase) column using a mobile phase consisting of Hexane: 2-propanol: Methanol: Acetic acid (80:10:...

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Autores principales:	RANE,VIPUL. P, PATIL,KIRAN. R, YEOLE,RAVINDRA. D, SHINDE,DEVANAND. B
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2013
Materias:	Metolazone Method development Enantiomeric separation Amylose based stationary phase Validation
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072013000100019
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spelling	oai:scielo:S0717-970720130001000192014-09-09ENANTIOMERIC LC SEPARATION OF METOLAZONE ON AMYLOSE BASED SORBENTRANE,VIPUL. PPATIL,KIRAN. RYEOLE,RAVINDRA. DSHINDE,DEVANAND. B Metolazone Method development Enantiomeric separation Amylose based stationary phase Validation A simple, rapid and robust LC method was developed and validated for the enantiomeric separation of metolazone. The enantiomers of metolazone were resolved on a Chiralpak AD-H (amylose based stationary phase) column using a mobile phase consisting of Hexane: 2-propanol: Methanol: Acetic acid (80:10:10:0.2, v/v) at a flow rate of 1.0 mLmin-1. The resolution between the enantiomers was found to be not less than 3.0 in optimized method. The presence of acetic acid in the mobile phase played an important role, in enhancing chromatographic efficiency and resolution between the enantiomers. The developed method was extensively validated and proved to be robust. The calibration curve for enantiomers showed excellent linearity over the concentration range of 5 μgmL-1 to 50 μgmL-1. The limit of detection and limit of quantification for enantiomers were 0.05 and 0.16 μgmL-1, respectively. The proposed method was found to be suitable and accurate for quantitative determination of enantiomers in bulk drug substance.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.58 n.1 20132013-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072013000100019en10.4067/S0717-97072013000100019
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Metolazone Method development Enantiomeric separation Amylose based stationary phase Validation
spellingShingle	Metolazone Method development Enantiomeric separation Amylose based stationary phase Validation RANE,VIPUL. P PATIL,KIRAN. R YEOLE,RAVINDRA. D SHINDE,DEVANAND. B ENANTIOMERIC LC SEPARATION OF METOLAZONE ON AMYLOSE BASED SORBENT
description	A simple, rapid and robust LC method was developed and validated for the enantiomeric separation of metolazone. The enantiomers of metolazone were resolved on a Chiralpak AD-H (amylose based stationary phase) column using a mobile phase consisting of Hexane: 2-propanol: Methanol: Acetic acid (80:10:10:0.2, v/v) at a flow rate of 1.0 mLmin-1. The resolution between the enantiomers was found to be not less than 3.0 in optimized method. The presence of acetic acid in the mobile phase played an important role, in enhancing chromatographic efficiency and resolution between the enantiomers. The developed method was extensively validated and proved to be robust. The calibration curve for enantiomers showed excellent linearity over the concentration range of 5 μgmL-1 to 50 μgmL-1. The limit of detection and limit of quantification for enantiomers were 0.05 and 0.16 μgmL-1, respectively. The proposed method was found to be suitable and accurate for quantitative determination of enantiomers in bulk drug substance.
author	RANE,VIPUL. P PATIL,KIRAN. R YEOLE,RAVINDRA. D SHINDE,DEVANAND. B
author_facet	RANE,VIPUL. P PATIL,KIRAN. R YEOLE,RAVINDRA. D SHINDE,DEVANAND. B
author_sort	RANE,VIPUL. P
title	ENANTIOMERIC LC SEPARATION OF METOLAZONE ON AMYLOSE BASED SORBENT
title_short	ENANTIOMERIC LC SEPARATION OF METOLAZONE ON AMYLOSE BASED SORBENT
title_full	ENANTIOMERIC LC SEPARATION OF METOLAZONE ON AMYLOSE BASED SORBENT
title_fullStr	ENANTIOMERIC LC SEPARATION OF METOLAZONE ON AMYLOSE BASED SORBENT
title_full_unstemmed	ENANTIOMERIC LC SEPARATION OF METOLAZONE ON AMYLOSE BASED SORBENT
title_sort	enantiomeric lc separation of metolazone on amylose based sorbent
publisher	Sociedad Chilena de Química
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072013000100019
work_keys_str_mv	AT ranevipulp enantiomericlcseparationofmetolazoneonamylosebasedsorbent AT patilkiranr enantiomericlcseparationofmetolazoneonamylosebasedsorbent AT yeoleravindrad enantiomericlcseparationofmetolazoneonamylosebasedsorbent AT shindedevanandb enantiomericlcseparationofmetolazoneonamylosebasedsorbent
_version_	1718445482204725248

ENANTIOMERIC LC SEPARATION OF METOLAZONE ON AMYLOSE BASED SORBENT

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