POLYETHYLENE GLYCOL EFFECT ON THE TRANSIENT AND STEADY STATE PHASES OF P-NITROPHENYLTRIMETHYL ACETATE HYDROLYSIS CATALYZED BY α-CHYMOTRYPSIN

The effect of polyethylene glycol (PEG) on the hydrolytic activity of α-chymotrypsin has been studied using p-nitrophenyltrimethyl acetate (PNTMA) as substrate. This substrate requires long times to reach the steady state condition, allowing the measurement by conventional methods of the fa...

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Auteurs principaux: CALDERÓN,CRISTIAN, LISSI,EDUARDO
Langue:English
Publié: Sociedad Chilena de Química 2013
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Accès en ligne:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072013000400033
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