POLYETHYLENE GLYCOL EFFECT ON THE TRANSIENT AND STEADY STATE PHASES OF P-NITROPHENYLTRIMETHYL ACETATE HYDROLYSIS CATALYZED BY α-CHYMOTRYPSIN

The effect of polyethylene glycol (PEG) on the hydrolytic activity of α-chymotrypsin has been studied using p-nitrophenyltrimethyl acetate (PNTMA) as substrate. This substrate requires long times to reach the steady state condition, allowing the measurement by conventional methods of the fa...

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Autores principales:	CALDERÓN,CRISTIAN, LISSI,EDUARDO
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2013
Materias:	Enzyme kinetics chymotrypsin p-nitrophenyl trimethylacetate poly-ethyleneglycol
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072013000400033
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spelling	oai:scielo:S0717-970720130004000332014-09-02POLYETHYLENE GLYCOL EFFECT ON THE TRANSIENT AND STEADY STATE PHASES OF P-NITROPHENYLTRIMETHYL ACETATE HYDROLYSIS CATALYZED BY α-CHYMOTRYPSINCALDERÓN,CRISTIANLISSI,EDUARDO Enzyme kinetics chymotrypsin p-nitrophenyl trimethylacetate poly-ethyleneglycol The effect of polyethylene glycol (PEG) on the hydrolytic activity of α-chymotrypsin has been studied using p-nitrophenyltrimethyl acetate (PNTMA) as substrate. This substrate requires long times to reach the steady state condition, allowing the measurement by conventional methods of the fast initial "burst" step present on the biphasic catalytic behaviour exhibited by α-chymotrypsin. The presence of PEG increases both the initial and steady state rates of the reaction. This increase was observed at all the substrate concentrations employed. A formal Michaelis-Menten treatment under steady state conditions shows that K M remains almost constant over the range of PEG concentrations studied (up to 20% w/v), whereas k cat moderately increases with the increment in PEG concentration. The results obtained in this work are interpreted in terms of the following mechanism:info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.58 n.4 20132013-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072013000400033en10.4067/S0717-97072013000400033
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Enzyme kinetics chymotrypsin p-nitrophenyl trimethylacetate poly-ethyleneglycol
spellingShingle	Enzyme kinetics chymotrypsin p-nitrophenyl trimethylacetate poly-ethyleneglycol CALDERÓN,CRISTIAN LISSI,EDUARDO POLYETHYLENE GLYCOL EFFECT ON THE TRANSIENT AND STEADY STATE PHASES OF P-NITROPHENYLTRIMETHYL ACETATE HYDROLYSIS CATALYZED BY α-CHYMOTRYPSIN
description	The effect of polyethylene glycol (PEG) on the hydrolytic activity of α-chymotrypsin has been studied using p-nitrophenyltrimethyl acetate (PNTMA) as substrate. This substrate requires long times to reach the steady state condition, allowing the measurement by conventional methods of the fast initial "burst" step present on the biphasic catalytic behaviour exhibited by α-chymotrypsin. The presence of PEG increases both the initial and steady state rates of the reaction. This increase was observed at all the substrate concentrations employed. A formal Michaelis-Menten treatment under steady state conditions shows that K M remains almost constant over the range of PEG concentrations studied (up to 20% w/v), whereas k cat moderately increases with the increment in PEG concentration. The results obtained in this work are interpreted in terms of the following mechanism:
author	CALDERÓN,CRISTIAN LISSI,EDUARDO
author_facet	CALDERÓN,CRISTIAN LISSI,EDUARDO
author_sort	CALDERÓN,CRISTIAN
title	POLYETHYLENE GLYCOL EFFECT ON THE TRANSIENT AND STEADY STATE PHASES OF P-NITROPHENYLTRIMETHYL ACETATE HYDROLYSIS CATALYZED BY α-CHYMOTRYPSIN
title_short	POLYETHYLENE GLYCOL EFFECT ON THE TRANSIENT AND STEADY STATE PHASES OF P-NITROPHENYLTRIMETHYL ACETATE HYDROLYSIS CATALYZED BY α-CHYMOTRYPSIN
title_full	POLYETHYLENE GLYCOL EFFECT ON THE TRANSIENT AND STEADY STATE PHASES OF P-NITROPHENYLTRIMETHYL ACETATE HYDROLYSIS CATALYZED BY α-CHYMOTRYPSIN
title_fullStr	POLYETHYLENE GLYCOL EFFECT ON THE TRANSIENT AND STEADY STATE PHASES OF P-NITROPHENYLTRIMETHYL ACETATE HYDROLYSIS CATALYZED BY α-CHYMOTRYPSIN
title_full_unstemmed	POLYETHYLENE GLYCOL EFFECT ON THE TRANSIENT AND STEADY STATE PHASES OF P-NITROPHENYLTRIMETHYL ACETATE HYDROLYSIS CATALYZED BY α-CHYMOTRYPSIN
title_sort	polyethylene glycol effect on the transient and steady state phases of p-nitrophenyltrimethyl acetate hydrolysis catalyzed by α-chymotrypsin
publisher	Sociedad Chilena de Química
publishDate	2013
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072013000400033
work_keys_str_mv	AT calderoncristian polyethyleneglycoleffectonthetransientandsteadystatephasesofpnitrophenyltrimethylacetatehydrolysiscatalyzedby945chymotrypsin AT lissieduardo polyethyleneglycoleffectonthetransientandsteadystatephasesofpnitrophenyltrimethylacetatehydrolysiscatalyzedby945chymotrypsin
_version_	1718445502084677632

POLYETHYLENE GLYCOL EFFECT ON THE TRANSIENT AND STEADY STATE PHASES OF P-NITROPHENYLTRIMETHYL ACETATE HYDROLYSIS CATALYZED BY &#945;-CHYMOTRYPSIN

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