POLYETHYLENE GLYCOL EFFECT ON THE TRANSIENT AND STEADY STATE PHASES OF P-NITROPHENYLTRIMETHYL ACETATE HYDROLYSIS CATALYZED BY α-CHYMOTRYPSIN
The effect of polyethylene glycol (PEG) on the hydrolytic activity of α-chymotrypsin has been studied using p-nitrophenyltrimethyl acetate (PNTMA) as substrate. This substrate requires long times to reach the steady state condition, allowing the measurement by conventional methods of the fa...
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Autores principales: | CALDERÓN,CRISTIAN, LISSI,EDUARDO |
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Lenguaje: | English |
Publicado: |
Sociedad Chilena de Química
2013
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Materias: | |
Acceso en línea: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072013000400033 |
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