IMPROVEMENT OF THE ANTIFUNGAL ACTIVITY AGAINST BOTRYTIS CINEREA OF SYRINGIC ACID, A PHENOLIC ACID FROM GRAPE POMACE

The aim of this study was to improve the antifungal activity against Botrytis cinerea of syringic acid by using the enzyme laccase to synthesize a heterodimeric compound by a coupling reaction with aniline. The synthesized heterodimer is a quinone-imine like compound (2,6-dimethoxy-4-(phenylimino)be...

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Autores principales:	MENDOZA,LEONORA, CASTRO,PAULO, MELO,RICARDO, CAMPOS,ANA MARÍA, ZÚÑIGA,GUSTAVO, GUERRERO,JUAN, COTORAS,MILENA
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2016
Materias:	Botrytis cinerea antifungal activity laccase 2,6-dimethoxy-4-(phenylimino)benzenone
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072016000300006
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spelling	oai:scielo:S0717-970720160003000062017-06-14IMPROVEMENT OF THE ANTIFUNGAL ACTIVITY AGAINST BOTRYTIS CINEREA OF SYRINGIC ACID, A PHENOLIC ACID FROM GRAPE POMACEMENDOZA,LEONORACASTRO,PAULOMELO,RICARDOCAMPOS,ANA MARÍAZÚÑIGA,GUSTAVOGUERRERO,JUANCOTORAS,MILENA Botrytis cinerea antifungal activity laccase 2,6-dimethoxy-4-(phenylimino)benzenone The aim of this study was to improve the antifungal activity against Botrytis cinerea of syringic acid by using the enzyme laccase to synthesize a heterodimeric compound by a coupling reaction with aniline. The synthesized heterodimer is a quinone-imine like compound (2,6-dimethoxy-4-(phenylimino)benzenone), which was characterized by using ¹H and 13C NMR, IR, and mass spectrometry. The antifungal activity of the heterodimeric compound against B. cinerea was determined in vitro and showed a higher antifungal effect than the substrates (syringic acid and aniline), inhibiting the mycelial growth with an IC50 value of 0.14mM and delayed 2 hours the conidial germination. Also, using the fluorochrome calcofluor white, cell wall damage was observed when B. cinerea was incubated with the compound. This is the first report on antifungal activity of this type of compound against B. cinerea.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.61 n.3 20162016-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072016000300006en10.4067/S0717-97072016000300006
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Botrytis cinerea antifungal activity laccase 2,6-dimethoxy-4-(phenylimino)benzenone
spellingShingle	Botrytis cinerea antifungal activity laccase 2,6-dimethoxy-4-(phenylimino)benzenone MENDOZA,LEONORA CASTRO,PAULO MELO,RICARDO CAMPOS,ANA MARÍA ZÚÑIGA,GUSTAVO GUERRERO,JUAN COTORAS,MILENA IMPROVEMENT OF THE ANTIFUNGAL ACTIVITY AGAINST BOTRYTIS CINEREA OF SYRINGIC ACID, A PHENOLIC ACID FROM GRAPE POMACE
description	The aim of this study was to improve the antifungal activity against Botrytis cinerea of syringic acid by using the enzyme laccase to synthesize a heterodimeric compound by a coupling reaction with aniline. The synthesized heterodimer is a quinone-imine like compound (2,6-dimethoxy-4-(phenylimino)benzenone), which was characterized by using ¹H and 13C NMR, IR, and mass spectrometry. The antifungal activity of the heterodimeric compound against B. cinerea was determined in vitro and showed a higher antifungal effect than the substrates (syringic acid and aniline), inhibiting the mycelial growth with an IC50 value of 0.14mM and delayed 2 hours the conidial germination. Also, using the fluorochrome calcofluor white, cell wall damage was observed when B. cinerea was incubated with the compound. This is the first report on antifungal activity of this type of compound against B. cinerea.
author	MENDOZA,LEONORA CASTRO,PAULO MELO,RICARDO CAMPOS,ANA MARÍA ZÚÑIGA,GUSTAVO GUERRERO,JUAN COTORAS,MILENA
author_facet	MENDOZA,LEONORA CASTRO,PAULO MELO,RICARDO CAMPOS,ANA MARÍA ZÚÑIGA,GUSTAVO GUERRERO,JUAN COTORAS,MILENA
author_sort	MENDOZA,LEONORA
title	IMPROVEMENT OF THE ANTIFUNGAL ACTIVITY AGAINST BOTRYTIS CINEREA OF SYRINGIC ACID, A PHENOLIC ACID FROM GRAPE POMACE
title_short	IMPROVEMENT OF THE ANTIFUNGAL ACTIVITY AGAINST BOTRYTIS CINEREA OF SYRINGIC ACID, A PHENOLIC ACID FROM GRAPE POMACE
title_full	IMPROVEMENT OF THE ANTIFUNGAL ACTIVITY AGAINST BOTRYTIS CINEREA OF SYRINGIC ACID, A PHENOLIC ACID FROM GRAPE POMACE
title_fullStr	IMPROVEMENT OF THE ANTIFUNGAL ACTIVITY AGAINST BOTRYTIS CINEREA OF SYRINGIC ACID, A PHENOLIC ACID FROM GRAPE POMACE
title_full_unstemmed	IMPROVEMENT OF THE ANTIFUNGAL ACTIVITY AGAINST BOTRYTIS CINEREA OF SYRINGIC ACID, A PHENOLIC ACID FROM GRAPE POMACE
title_sort	improvement of the antifungal activity against botrytis cinerea of syringic acid, a phenolic acid from grape pomace
publisher	Sociedad Chilena de Química
publishDate	2016
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072016000300006
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IMPROVEMENT OF THE ANTIFUNGAL ACTIVITY AGAINST BOTRYTIS CINEREA OF SYRINGIC ACID, A PHENOLIC ACID FROM GRAPE POMACE

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