DEVELOPMENT AND CHARACTERIZATION OF A SENSOR BASED ON CARBON NANOFIBERS: APPLICATION TO ACETAZOLAMIDE DETERMINATION IN PHARMACEUTICALS AND BIOLOGICAL FLUIDS

ABSTRACT Acetazolamide (ACZ) is a carbonic anhydrase inhibitor that exhibits diuretic activity. In medicine, it is principally used in open-angle glaucoma, for prevention or amelioration of symptoms associated with acute high-altitude sickness and as an adjunct to other anticonvulsants in centrencep...

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Autores principales:	Mundaca-Uribe,Rodolfo, Diego,Marta de, Henríquez-Aedo,Karem, Aranda,Mario, Peña-Farfal,Carlos
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2019
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072019000104382
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spelling	oai:scielo:S0717-970720190001043822019-04-11DEVELOPMENT AND CHARACTERIZATION OF A SENSOR BASED ON CARBON NANOFIBERS: APPLICATION TO ACETAZOLAMIDE DETERMINATION IN PHARMACEUTICALS AND BIOLOGICAL FLUIDSMundaca-Uribe,RodolfoDiego,Marta deHenríquez-Aedo,KaremAranda,MarioPeña-Farfal,CarlosABSTRACT Acetazolamide (ACZ) is a carbonic anhydrase inhibitor that exhibits diuretic activity. In medicine, it is principally used in open-angle glaucoma, for prevention or amelioration of symptoms associated with acute high-altitude sickness and as an adjunct to other anticonvulsants in centrencephalic epilepsies. Furthermore, ACZ is sometimes used by athletes to mask the presence of doping substances, so its use has been banned in competitions. The aim of this work was the development of a sensor to determine ACZ in pharmaceuticals, as quality control way, and serum, for clinical and doping analyzes. The sensor has been developed modifying a glassy carbon electrode with carbon nanofibers. The electrochemical characterization of the sensor was performed by cyclic voltammetry using the redox mediator potassium ferrocyanide. ACZ electrochemical behavior was studied as well. Measurements were performed in a conventional three electrodes cell using differential pulse voltammetry. The influence of some experimental variables involved in the preparation and performance of the sensor –amount of modifier, supporting electrolyte, pH, scan rate and pulse amplitude– were optimized. This methodology provided a linear calibration plot for ACZ in the 1 – 17 µM concentration range. Furthermore, the proposed sensor exhibited suitable analytical properties, with detection and quantification limits of 0.06 µM and 0.2 µM respectively; analytical sensitivity of 1.142 µA/µM, repeatability of 0.72 %, and it was applied to determine ACZ in pharmaceuticals (tablets) and spiked human serum, being an excellent tool to perform quality control and antidoping control.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.64 n.1 20192019-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072019000104382en10.4067/s0717-97072019000104382
institution	Scielo Chile
collection	Scielo Chile
language	English
description	ABSTRACT Acetazolamide (ACZ) is a carbonic anhydrase inhibitor that exhibits diuretic activity. In medicine, it is principally used in open-angle glaucoma, for prevention or amelioration of symptoms associated with acute high-altitude sickness and as an adjunct to other anticonvulsants in centrencephalic epilepsies. Furthermore, ACZ is sometimes used by athletes to mask the presence of doping substances, so its use has been banned in competitions. The aim of this work was the development of a sensor to determine ACZ in pharmaceuticals, as quality control way, and serum, for clinical and doping analyzes. The sensor has been developed modifying a glassy carbon electrode with carbon nanofibers. The electrochemical characterization of the sensor was performed by cyclic voltammetry using the redox mediator potassium ferrocyanide. ACZ electrochemical behavior was studied as well. Measurements were performed in a conventional three electrodes cell using differential pulse voltammetry. The influence of some experimental variables involved in the preparation and performance of the sensor –amount of modifier, supporting electrolyte, pH, scan rate and pulse amplitude– were optimized. This methodology provided a linear calibration plot for ACZ in the 1 – 17 µM concentration range. Furthermore, the proposed sensor exhibited suitable analytical properties, with detection and quantification limits of 0.06 µM and 0.2 µM respectively; analytical sensitivity of 1.142 µA/µM, repeatability of 0.72 %, and it was applied to determine ACZ in pharmaceuticals (tablets) and spiked human serum, being an excellent tool to perform quality control and antidoping control.
author	Mundaca-Uribe,Rodolfo Diego,Marta de Henríquez-Aedo,Karem Aranda,Mario Peña-Farfal,Carlos
spellingShingle	Mundaca-Uribe,Rodolfo Diego,Marta de Henríquez-Aedo,Karem Aranda,Mario Peña-Farfal,Carlos DEVELOPMENT AND CHARACTERIZATION OF A SENSOR BASED ON CARBON NANOFIBERS: APPLICATION TO ACETAZOLAMIDE DETERMINATION IN PHARMACEUTICALS AND BIOLOGICAL FLUIDS
author_facet	Mundaca-Uribe,Rodolfo Diego,Marta de Henríquez-Aedo,Karem Aranda,Mario Peña-Farfal,Carlos
author_sort	Mundaca-Uribe,Rodolfo
title	DEVELOPMENT AND CHARACTERIZATION OF A SENSOR BASED ON CARBON NANOFIBERS: APPLICATION TO ACETAZOLAMIDE DETERMINATION IN PHARMACEUTICALS AND BIOLOGICAL FLUIDS
title_short	DEVELOPMENT AND CHARACTERIZATION OF A SENSOR BASED ON CARBON NANOFIBERS: APPLICATION TO ACETAZOLAMIDE DETERMINATION IN PHARMACEUTICALS AND BIOLOGICAL FLUIDS
title_full	DEVELOPMENT AND CHARACTERIZATION OF A SENSOR BASED ON CARBON NANOFIBERS: APPLICATION TO ACETAZOLAMIDE DETERMINATION IN PHARMACEUTICALS AND BIOLOGICAL FLUIDS
title_fullStr	DEVELOPMENT AND CHARACTERIZATION OF A SENSOR BASED ON CARBON NANOFIBERS: APPLICATION TO ACETAZOLAMIDE DETERMINATION IN PHARMACEUTICALS AND BIOLOGICAL FLUIDS
title_full_unstemmed	DEVELOPMENT AND CHARACTERIZATION OF A SENSOR BASED ON CARBON NANOFIBERS: APPLICATION TO ACETAZOLAMIDE DETERMINATION IN PHARMACEUTICALS AND BIOLOGICAL FLUIDS
title_sort	development and characterization of a sensor based on carbon nanofibers: application to acetazolamide determination in pharmaceuticals and biological fluids
publisher	Sociedad Chilena de Química
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072019000104382
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DEVELOPMENT AND CHARACTERIZATION OF A SENSOR BASED ON CARBON NANOFIBERS: APPLICATION TO ACETAZOLAMIDE DETERMINATION IN PHARMACEUTICALS AND BIOLOGICAL FLUIDS

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