LC-MS/MS METHOD FOR L-DOPA QUANTIFICATION IN DIFFERENT TISSUES OF VICIA FABA

ABSTRACT A rapid, sensitive, precise and accurate liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed for levo 3,4-dihydroxyphenylalanine (L-DOPA) determination in Vicia faba during different growth stages. The method applied a simple sample preparation step followed by a...

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Autores principales:	Pavón-Pérez,Jessy, Oviedo,Claudia A., Elso-Freudenberg,Mhartyn, Henríquez-Aedo,Karem, Aranda,Mario
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2019
Materias:	Mass spectrometry L-DOPA Vicia faba LC-MS/MS
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072019000404651
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spelling	oai:scielo:S0717-970720190004046512020-02-03LC-MS/MS METHOD FOR L-DOPA QUANTIFICATION IN DIFFERENT TISSUES OF VICIA FABAPavón-Pérez,JessyOviedo,Claudia A.Elso-Freudenberg,MhartynHenríquez-Aedo,KaremAranda,Mario Mass spectrometry L-DOPA Vicia faba LC-MS/MS ABSTRACT A rapid, sensitive, precise and accurate liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed for levo 3,4-dihydroxyphenylalanine (L-DOPA) determination in Vicia faba during different growth stages. The method applied a simple sample preparation step followed by a chromatographic separation on a Kinetex XB Core-Shell C18 (100 mm x 4.6 mm, 2.6 µm) column, using a mixture of ultrapure pure water (A) with 0.5 % (v/v) formic acid and methanol (B) as mobile phase. Analysis of L-DOPA was carried out by MS/MS applying a Multiple Reaction Monitoring (MRM) method using the transition m/z 198 → m/z 152. This LC-MS/MS method allowed a well-resolved detection of L-DOPA in ca. 2 min within 6 min run. Method was validated showing a linear range from 0.05 to 10 mg L-1 (R2 = 0.99); repeatability showed RSD value of 1.40%. Recoveries ranged from 94.14 to 116.62% with RSD values ≤ 5.66% and detection and quantification limits were 0.01 and 0.05 mg L-1, respectively. Applying this validated method, L-DOPA was determined in Vicia faba samples to determined its tissue distribution. As expected, a broad range of L-DOPA content finding the lowest content in seeds (4.72±2.12 mg g-1) and the highest in sprouts (133.60±5.65 mg g-1).info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.64 n.4 20192019-12-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072019000404651en10.4067/S0717-97072019000404651
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Mass spectrometry L-DOPA Vicia faba LC-MS/MS
spellingShingle	Mass spectrometry L-DOPA Vicia faba LC-MS/MS Pavón-Pérez,Jessy Oviedo,Claudia A. Elso-Freudenberg,Mhartyn Henríquez-Aedo,Karem Aranda,Mario LC-MS/MS METHOD FOR L-DOPA QUANTIFICATION IN DIFFERENT TISSUES OF VICIA FABA
description	ABSTRACT A rapid, sensitive, precise and accurate liquid chromatography tandem mass spectrometry (LC-MS/MS) method was developed for levo 3,4-dihydroxyphenylalanine (L-DOPA) determination in Vicia faba during different growth stages. The method applied a simple sample preparation step followed by a chromatographic separation on a Kinetex XB Core-Shell C18 (100 mm x 4.6 mm, 2.6 µm) column, using a mixture of ultrapure pure water (A) with 0.5 % (v/v) formic acid and methanol (B) as mobile phase. Analysis of L-DOPA was carried out by MS/MS applying a Multiple Reaction Monitoring (MRM) method using the transition m/z 198 → m/z 152. This LC-MS/MS method allowed a well-resolved detection of L-DOPA in ca. 2 min within 6 min run. Method was validated showing a linear range from 0.05 to 10 mg L-1 (R2 = 0.99); repeatability showed RSD value of 1.40%. Recoveries ranged from 94.14 to 116.62% with RSD values ≤ 5.66% and detection and quantification limits were 0.01 and 0.05 mg L-1, respectively. Applying this validated method, L-DOPA was determined in Vicia faba samples to determined its tissue distribution. As expected, a broad range of L-DOPA content finding the lowest content in seeds (4.72±2.12 mg g-1) and the highest in sprouts (133.60±5.65 mg g-1).
author	Pavón-Pérez,Jessy Oviedo,Claudia A. Elso-Freudenberg,Mhartyn Henríquez-Aedo,Karem Aranda,Mario
author_facet	Pavón-Pérez,Jessy Oviedo,Claudia A. Elso-Freudenberg,Mhartyn Henríquez-Aedo,Karem Aranda,Mario
author_sort	Pavón-Pérez,Jessy
title	LC-MS/MS METHOD FOR L-DOPA QUANTIFICATION IN DIFFERENT TISSUES OF VICIA FABA
title_short	LC-MS/MS METHOD FOR L-DOPA QUANTIFICATION IN DIFFERENT TISSUES OF VICIA FABA
title_full	LC-MS/MS METHOD FOR L-DOPA QUANTIFICATION IN DIFFERENT TISSUES OF VICIA FABA
title_fullStr	LC-MS/MS METHOD FOR L-DOPA QUANTIFICATION IN DIFFERENT TISSUES OF VICIA FABA
title_full_unstemmed	LC-MS/MS METHOD FOR L-DOPA QUANTIFICATION IN DIFFERENT TISSUES OF VICIA FABA
title_sort	lc-ms/ms method for l-dopa quantification in different tissues of vicia faba
publisher	Sociedad Chilena de Química
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072019000404651
work_keys_str_mv	AT pavonperezjessy lcmsmsmethodforldopaquantificationindifferenttissuesofviciafaba AT oviedoclaudiaa lcmsmsmethodforldopaquantificationindifferenttissuesofviciafaba AT elsofreudenbergmhartyn lcmsmsmethodforldopaquantificationindifferenttissuesofviciafaba AT henriquezaedokarem lcmsmsmethodforldopaquantificationindifferenttissuesofviciafaba AT arandamario lcmsmsmethodforldopaquantificationindifferenttissuesofviciafaba
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LC-MS/MS METHOD FOR L-DOPA QUANTIFICATION IN DIFFERENT TISSUES OF VICIA FABA

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