EFFECT OF in vitro DIGESTION GASTROINTESTINAL OF THE EXTRACT AQUEOU OF LEAVES OF Ugni molinae , ON THE VIABILITY OF COLORECTAL CANCER CELLS

ABSTRACT Aim: This work is aimed to the study the effect of aqueous extracts obtained from leaves of Ugni molinae and their respective products of in vitro gastrointestinal digestion on the viability of colon cancer cells. Methods: Ugni molinae leaves were used to prepare a 1%, aqueous extract and...

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Autores principales:	Avello,Marcia, Torres,Eillen, Carvajal,Romina I., Pastene,Edgar
Lenguaje:	English
Publicado:	Sociedad Chilena de Química 2021
Materias:	Digestion gastrointestinal Viability Caco-2 Ugni molinae
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072021000305268
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spelling	oai:scielo:S0717-970720210003052682021-10-14EFFECT OF in vitro DIGESTION GASTROINTESTINAL OF THE EXTRACT AQUEOU OF LEAVES OF Ugni molinae , ON THE VIABILITY OF COLORECTAL CANCER CELLSAvello,MarciaTorres,EillenCarvajal,Romina I.Pastene,Edgar Digestion gastrointestinal Viability Caco-2 Ugni molinae ABSTRACT Aim: This work is aimed to the study the effect of aqueous extracts obtained from leaves of Ugni molinae and their respective products of in vitro gastrointestinal digestion on the viability of colon cancer cells. Methods: Ugni molinae leaves were used to prepare a 1%, aqueous extract and with this, an in vitro gastrointestinal digestion was performed, obtaining, fraction products of digestion gastrointestinal (Dgi) and fraction residue. this fraction was identified through liquid chromatography (HPLC-UV) and liquid chromatography-mass spectrometry (HPLC-MS), and then these samples were evaluated in different viability tests, such as trypan blue exclusion, metabolic activity 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and cytotoxicity by lactate dehydrogenase (LDH), on colorectal cancer cells (CaCo-2) and human embryonic kidney 293 cells (HEK) for a period of 24 and 48 hours. Results and discussion: The aqueous extracts of leaves of Ugni molinae and their digestion fractions caused inhibition of the viability of Caco-2 cells. In most cases, the effect was concentration-dependent and in particular, the only compound that generated inhibition of the viability in all trials corresponded to the Dgi fraction. Conclusion: The results are favorable, since it is finally is this product that would act in the gastrointestinal tract, interacting with the target cells. These results are important since in most cases, these compounds had effects on cancer cells Caco-2 and not on HEK cells at the same concentrations used in each trial.info:eu-repo/semantics/openAccessSociedad Chilena de QuímicaJournal of the Chilean Chemical Society v.66 n.3 20212021-09-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072021000305268en10.4067/S0717-97072021000305268
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Digestion gastrointestinal Viability Caco-2 Ugni molinae
spellingShingle	Digestion gastrointestinal Viability Caco-2 Ugni molinae Avello,Marcia Torres,Eillen Carvajal,Romina I. Pastene,Edgar EFFECT OF in vitro DIGESTION GASTROINTESTINAL OF THE EXTRACT AQUEOU OF LEAVES OF Ugni molinae , ON THE VIABILITY OF COLORECTAL CANCER CELLS
description	ABSTRACT Aim: This work is aimed to the study the effect of aqueous extracts obtained from leaves of Ugni molinae and their respective products of in vitro gastrointestinal digestion on the viability of colon cancer cells. Methods: Ugni molinae leaves were used to prepare a 1%, aqueous extract and with this, an in vitro gastrointestinal digestion was performed, obtaining, fraction products of digestion gastrointestinal (Dgi) and fraction residue. this fraction was identified through liquid chromatography (HPLC-UV) and liquid chromatography-mass spectrometry (HPLC-MS), and then these samples were evaluated in different viability tests, such as trypan blue exclusion, metabolic activity 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and cytotoxicity by lactate dehydrogenase (LDH), on colorectal cancer cells (CaCo-2) and human embryonic kidney 293 cells (HEK) for a period of 24 and 48 hours. Results and discussion: The aqueous extracts of leaves of Ugni molinae and their digestion fractions caused inhibition of the viability of Caco-2 cells. In most cases, the effect was concentration-dependent and in particular, the only compound that generated inhibition of the viability in all trials corresponded to the Dgi fraction. Conclusion: The results are favorable, since it is finally is this product that would act in the gastrointestinal tract, interacting with the target cells. These results are important since in most cases, these compounds had effects on cancer cells Caco-2 and not on HEK cells at the same concentrations used in each trial.
author	Avello,Marcia Torres,Eillen Carvajal,Romina I. Pastene,Edgar
author_facet	Avello,Marcia Torres,Eillen Carvajal,Romina I. Pastene,Edgar
author_sort	Avello,Marcia
title	EFFECT OF in vitro DIGESTION GASTROINTESTINAL OF THE EXTRACT AQUEOU OF LEAVES OF Ugni molinae , ON THE VIABILITY OF COLORECTAL CANCER CELLS
title_short	EFFECT OF in vitro DIGESTION GASTROINTESTINAL OF THE EXTRACT AQUEOU OF LEAVES OF Ugni molinae , ON THE VIABILITY OF COLORECTAL CANCER CELLS
title_full	EFFECT OF in vitro DIGESTION GASTROINTESTINAL OF THE EXTRACT AQUEOU OF LEAVES OF Ugni molinae , ON THE VIABILITY OF COLORECTAL CANCER CELLS
title_fullStr	EFFECT OF in vitro DIGESTION GASTROINTESTINAL OF THE EXTRACT AQUEOU OF LEAVES OF Ugni molinae , ON THE VIABILITY OF COLORECTAL CANCER CELLS
title_full_unstemmed	EFFECT OF in vitro DIGESTION GASTROINTESTINAL OF THE EXTRACT AQUEOU OF LEAVES OF Ugni molinae , ON THE VIABILITY OF COLORECTAL CANCER CELLS
title_sort	effect of in vitro digestion gastrointestinal of the extract aqueou of leaves of ugni molinae , on the viability of colorectal cancer cells
publisher	Sociedad Chilena de Química
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-97072021000305268
work_keys_str_mv	AT avellomarcia effectofinvitrodigestiongastrointestinaloftheextractaqueouofleavesofugnimolinaeontheviabilityofcolorectalcancercells AT torreseillen effectofinvitrodigestiongastrointestinaloftheextractaqueouofleavesofugnimolinaeontheviabilityofcolorectalcancercells AT carvajalrominai effectofinvitrodigestiongastrointestinaloftheextractaqueouofleavesofugnimolinaeontheviabilityofcolorectalcancercells AT pasteneedgar effectofinvitrodigestiongastrointestinaloftheextractaqueouofleavesofugnimolinaeontheviabilityofcolorectalcancercells
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EFFECT OF in vitro DIGESTION GASTROINTESTINAL OF THE EXTRACT AQUEOU OF LEAVES OF Ugni molinae , ON THE VIABILITY OF COLORECTAL CANCER CELLS

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