Efficient protocols for the extraction of microbial DNA from the rhizosphere of hydrophilic forests in Chile

Efficient protocols for the extraction of microbial DNA from the rhizosphere of hydrophilic forests in Chile

A lysis buffer-based protocol (Protocol BA), a modified lysis buffer-based protocol (Protocol BA Mod) and a commercial extraction kit (Protocol PS Kit) (Power Soil, Mo bio Laboratories, CA USA) were each evaluated for their ability to produce high-quality DNA with yields sufficient to allow its use...

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Auteurs principaux: Cáceres,Pablo, Cordero,Cecilia, González,Gloria, Quiroz,Karla, Bobadilla,Juan C, Bravo,Carmen, Caligari,Peter D.S, Carrasco,Basilio, García-Gonzales,Rolando
Langue:English
Publié: Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal 2012
Sujets:
hydrophilic forests
16S ribosomal RNA
PCR
rhizosphere
soil DNA extraction
Accès en ligne:http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000300018
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http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000300018

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