Protein enrichment of Opuntia ficus-indica using Kluyveromyces marxianus in solid-state fermentation

Abstract Opuntia ficus-indica is used in animal feed but has a low protein content (above 4%) in comparison to high-quality forage (above 16%). It is necessary to develop technologies that improve the nutrimental value of Opuntia ficus-indica through the process of solid-state fermentation (SSF). Th...

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Autores principales:	Herrera,Esperanza, Murillo,Manuel, Berumen,Leslie, Soto-Cruz,Nicolas O., Páez-Lerma,Jesús B.
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal 2017
Materias:	Fermentation nutritional quality protein Opuntia yeast
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202017000200113
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spelling	oai:scielo:S0718-162020170002001132017-09-26Protein enrichment of Opuntia ficus-indica using Kluyveromyces marxianus in solid-state fermentationHerrera,EsperanzaMurillo,ManuelBerumen,LeslieSoto-Cruz,Nicolas O.Páez-Lerma,Jesús B. Fermentation nutritional quality protein Opuntia yeast Abstract Opuntia ficus-indica is used in animal feed but has a low protein content (above 4%) in comparison to high-quality forage (above 16%). It is necessary to develop technologies that improve the nutrimental value of Opuntia ficus-indica through the process of solid-state fermentation (SSF). Therefore, the objective of this study was to evaluate the changes in the chemical composition of Opuntia ficus-indica during the SSF with the yeast Kluyveromyces marxianus ITD00262. Kluyveromyces marxianus ITD00262 was grown in glucose, peptone, yeast (GPY) broth, at 28 °C and pH 4.8 for 12 h. Opuntia ficusindica was incubated in SSF at 28 °C without inoculation (T1) or inoculated with K. marxianus ITD00262 (T2). Fermented samples of Opuntia ficus-indica were dried, and the dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), lignin (LIGN) and in vitro dry matter digestibility (IVDMD) contents were ascertained. Data were analyzed with a completely randomized design with a factorial arrangement of 2×6. A decrease of 11% in DM was observed for T1 compared with T2 at 48 h of fermentation. A 300% increase in PC content was observed for T2 at 120 h of fermentation. The DMIVD was increased by 3%, whereas the values of NDF and ADF decreased by 18% in T2. According to our results, it is confirmed that the use of K. marxianus in the SSF of Opuntia ficus-indica has a positive effect and improves the nutrimental quality of this forage.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería ForestalCiencia e investigación agraria v.44 n.2 20172017-01-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202017000200113en10.7764/rcia.v44i2.1767
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Fermentation nutritional quality protein Opuntia yeast
spellingShingle	Fermentation nutritional quality protein Opuntia yeast Herrera,Esperanza Murillo,Manuel Berumen,Leslie Soto-Cruz,Nicolas O. Páez-Lerma,Jesús B. Protein enrichment of Opuntia ficus-indica using Kluyveromyces marxianus in solid-state fermentation
description	Abstract Opuntia ficus-indica is used in animal feed but has a low protein content (above 4%) in comparison to high-quality forage (above 16%). It is necessary to develop technologies that improve the nutrimental value of Opuntia ficus-indica through the process of solid-state fermentation (SSF). Therefore, the objective of this study was to evaluate the changes in the chemical composition of Opuntia ficus-indica during the SSF with the yeast Kluyveromyces marxianus ITD00262. Kluyveromyces marxianus ITD00262 was grown in glucose, peptone, yeast (GPY) broth, at 28 °C and pH 4.8 for 12 h. Opuntia ficusindica was incubated in SSF at 28 °C without inoculation (T1) or inoculated with K. marxianus ITD00262 (T2). Fermented samples of Opuntia ficus-indica were dried, and the dry matter (DM), crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), lignin (LIGN) and in vitro dry matter digestibility (IVDMD) contents were ascertained. Data were analyzed with a completely randomized design with a factorial arrangement of 2×6. A decrease of 11% in DM was observed for T1 compared with T2 at 48 h of fermentation. A 300% increase in PC content was observed for T2 at 120 h of fermentation. The DMIVD was increased by 3%, whereas the values of NDF and ADF decreased by 18% in T2. According to our results, it is confirmed that the use of K. marxianus in the SSF of Opuntia ficus-indica has a positive effect and improves the nutrimental quality of this forage.
author	Herrera,Esperanza Murillo,Manuel Berumen,Leslie Soto-Cruz,Nicolas O. Páez-Lerma,Jesús B.
author_facet	Herrera,Esperanza Murillo,Manuel Berumen,Leslie Soto-Cruz,Nicolas O. Páez-Lerma,Jesús B.
author_sort	Herrera,Esperanza
title	Protein enrichment of Opuntia ficus-indica using Kluyveromyces marxianus in solid-state fermentation
title_short	Protein enrichment of Opuntia ficus-indica using Kluyveromyces marxianus in solid-state fermentation
title_full	Protein enrichment of Opuntia ficus-indica using Kluyveromyces marxianus in solid-state fermentation
title_fullStr	Protein enrichment of Opuntia ficus-indica using Kluyveromyces marxianus in solid-state fermentation
title_full_unstemmed	Protein enrichment of Opuntia ficus-indica using Kluyveromyces marxianus in solid-state fermentation
title_sort	protein enrichment of opuntia ficus-indica using kluyveromyces marxianus in solid-state fermentation
publisher	Pontificia Universidad Católica de Chile. Facultad de Agronomía e Ingeniería Forestal
publishDate	2017
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202017000200113
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Protein enrichment of Opuntia ficus-indica using Kluyveromyces marxianus in solid-state fermentation

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