Effects of pH and ionic strength on the protease activity of gastric extracts from the Coho salmon Oncorhynchus kisutch

ABSTRACT The activity of fish acid proteases is strongly dependent on the pH, and many basic and applied works had focused on researching the pH-profile of digestive proteases using different buffered media. Nevertheless, enzymatic activity is also known to be affected by the ionic strength (IS) of...

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Autores principales:	Márquez,Lorenzo, Hernández,Adrián J., Carvalho,Edison S.M., Morales,Gabriel A., Dantagnan,Patricio, Díaz,Manuel, Moyano,Francisco J.
Lenguaje:	English
Publicado:	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar 2019
Materias:	Oncorhynchus kisutch Coho salmon gastric proteases enzymatic activity pH ionic strength
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2019000500860
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spelling	oai:scielo:S0718-560X20190005008602019-12-05Effects of pH and ionic strength on the protease activity of gastric extracts from the Coho salmon Oncorhynchus kisutchMárquez,LorenzoHernández,Adrián J.Carvalho,Edison S.M.Morales,Gabriel A.Dantagnan,PatricioDíaz,ManuelMoyano,Francisco J. Oncorhynchus kisutch Coho salmon gastric proteases enzymatic activity pH ionic strength ABSTRACT The activity of fish acid proteases is strongly dependent on the pH, and many basic and applied works had focused on researching the pH-profile of digestive proteases using different buffered media. Nevertheless, enzymatic activity is also known to be affected by the ionic strength (IS) of the medium. The present work is aimed at researching the combined effects of pH (2.5, 3.0, 3.5, 3.75 and 4.0) and IS (50, 100 and 200 mM) on the protease activity of gastric extracts of juvenile Coho salmon Oncorhynchus kisutch. It is concluded that the activity of Coho salmon gastric proteases is maximal at pH 2.5-3.0, drops towards pH 4.0 irrespective of ionic strength, and they showed a lower activity at IS 50 mM in comparison to IS 100 or 200 mM. The present results point out to the convenience of considering the effect of ionic strength when measuring the activity of fish gastric proteases.info:eu-repo/semantics/openAccessPontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del MarLatin american journal of aquatic research v.47 n.5 20192019-11-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2019000500860en10.3856/vol47-issue5-fulltext-16
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Oncorhynchus kisutch Coho salmon gastric proteases enzymatic activity pH ionic strength
spellingShingle	Oncorhynchus kisutch Coho salmon gastric proteases enzymatic activity pH ionic strength Márquez,Lorenzo Hernández,Adrián J. Carvalho,Edison S.M. Morales,Gabriel A. Dantagnan,Patricio Díaz,Manuel Moyano,Francisco J. Effects of pH and ionic strength on the protease activity of gastric extracts from the Coho salmon Oncorhynchus kisutch
description	ABSTRACT The activity of fish acid proteases is strongly dependent on the pH, and many basic and applied works had focused on researching the pH-profile of digestive proteases using different buffered media. Nevertheless, enzymatic activity is also known to be affected by the ionic strength (IS) of the medium. The present work is aimed at researching the combined effects of pH (2.5, 3.0, 3.5, 3.75 and 4.0) and IS (50, 100 and 200 mM) on the protease activity of gastric extracts of juvenile Coho salmon Oncorhynchus kisutch. It is concluded that the activity of Coho salmon gastric proteases is maximal at pH 2.5-3.0, drops towards pH 4.0 irrespective of ionic strength, and they showed a lower activity at IS 50 mM in comparison to IS 100 or 200 mM. The present results point out to the convenience of considering the effect of ionic strength when measuring the activity of fish gastric proteases.
author	Márquez,Lorenzo Hernández,Adrián J. Carvalho,Edison S.M. Morales,Gabriel A. Dantagnan,Patricio Díaz,Manuel Moyano,Francisco J.
author_facet	Márquez,Lorenzo Hernández,Adrián J. Carvalho,Edison S.M. Morales,Gabriel A. Dantagnan,Patricio Díaz,Manuel Moyano,Francisco J.
author_sort	Márquez,Lorenzo
title	Effects of pH and ionic strength on the protease activity of gastric extracts from the Coho salmon Oncorhynchus kisutch
title_short	Effects of pH and ionic strength on the protease activity of gastric extracts from the Coho salmon Oncorhynchus kisutch
title_full	Effects of pH and ionic strength on the protease activity of gastric extracts from the Coho salmon Oncorhynchus kisutch
title_fullStr	Effects of pH and ionic strength on the protease activity of gastric extracts from the Coho salmon Oncorhynchus kisutch
title_full_unstemmed	Effects of pH and ionic strength on the protease activity of gastric extracts from the Coho salmon Oncorhynchus kisutch
title_sort	effects of ph and ionic strength on the protease activity of gastric extracts from the coho salmon oncorhynchus kisutch
publisher	Pontificia Universidad Católica de Valparaíso. Facultad de Recursos Naturales. Escuela de Ciencias del Mar
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-560X2019000500860
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_version_	1714205229475430400

Effects of pH and ionic strength on the protease activity of gastric extracts from the Coho salmon Oncorhynchus kisutch

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