Changes in phytochemical content and antioxidant activity during inflorescence development in broccoli

ABSTRACT This study was performed to monitor glucosinolate (GSL) profile, total phenol, ascorbic acid, total flavonoid contents, and antioxidant activity in florets of six broccoli (Brassica oleracea L. var. italica Plenck) genotypes at three different inflorescence developmental stages. The level o...

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Autores principales:	Bhandari,Shiva Ram, Kwak,Jung-Ho, Jo,Jung Su, Lee,Jun Gu
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2019
Materias:	Antioxidant activities ascorbic acid Brassica oleracea var. italica broccoli floret developmental stage glucosinolate phenolics.
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392019000100036
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spelling	oai:scielo:S0718-583920190001000362019-02-19Changes in phytochemical content and antioxidant activity during inflorescence development in broccoliBhandari,Shiva RamKwak,Jung-HoJo,Jung SuLee,Jun Gu Antioxidant activities ascorbic acid Brassica oleracea var. italica broccoli floret developmental stage glucosinolate phenolics. ABSTRACT This study was performed to monitor glucosinolate (GSL) profile, total phenol, ascorbic acid, total flavonoid contents, and antioxidant activity in florets of six broccoli (Brassica oleracea L. var. italica Plenck) genotypes at three different inflorescence developmental stages. The level of phytochemicals and antioxidant activities varied significantly among genotypes and developmental stages. Out of the eight GSLs identified in this study, only five were present in all genotypes and their respective developmental stages. Glucoraphanin (GRA), a major GSL, was significantly increased towards the later stages of development (1.41 μg g-1 in immature stage of ‘Koyoshi’ to 10.57 μg g-1 in commercial stage of ‘09FA- M295’), whereas glucobrassicin (BRA) showed a reverse accumulation pattern with the highest value at the immature stage (5.10-7.97 μg g-1). Other minor GSLs varied depending on the genotype. Total phenolic and ascorbic acid content increased significantly as maturity progressed in all the genotypes with a relatively higher increment observed in ascorbic acid (65.8%-100.9%) than phenolic content (10.2%-31.2%). Flavonoid content, on the other hand, showed a cultivar- dependent accumulation pattern throughout the developmental stages. However, although antioxidant activity increased with inflorescence development, the increment was higher and lower than in total phenol and ascorbic acid content, respectively. The highest value for GRA (10.57 μg g-1), ascorbic acid (502.3 mg 100 g-1), total phenol (523.7 mg 100 g-1) and antioxidant activities was obtained for ‘09FA-M295’ at the commercial stage. Taken together, these results indicate that phytochemicals in broccoli have a differential accumulation pattern during inflorescence development. Regardless of genotypes and inflorescence developmental stage, total phenolic content showed the highest correlation with antioxidant activity (r = 0.779), followed by ascorbic acid (r = 0.674) and flavonoid content (r = 0.602**), whereas total GSLs showed a nonsignificant correlation with antioxidant activity.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.79 n.1 20192019-03-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392019000100036en10.4067/S0718-58392019000100036
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Antioxidant activities ascorbic acid Brassica oleracea var. italica broccoli floret developmental stage glucosinolate phenolics.
spellingShingle	Antioxidant activities ascorbic acid Brassica oleracea var. italica broccoli floret developmental stage glucosinolate phenolics. Bhandari,Shiva Ram Kwak,Jung-Ho Jo,Jung Su Lee,Jun Gu Changes in phytochemical content and antioxidant activity during inflorescence development in broccoli
description	ABSTRACT This study was performed to monitor glucosinolate (GSL) profile, total phenol, ascorbic acid, total flavonoid contents, and antioxidant activity in florets of six broccoli (Brassica oleracea L. var. italica Plenck) genotypes at three different inflorescence developmental stages. The level of phytochemicals and antioxidant activities varied significantly among genotypes and developmental stages. Out of the eight GSLs identified in this study, only five were present in all genotypes and their respective developmental stages. Glucoraphanin (GRA), a major GSL, was significantly increased towards the later stages of development (1.41 μg g-1 in immature stage of ‘Koyoshi’ to 10.57 μg g-1 in commercial stage of ‘09FA- M295’), whereas glucobrassicin (BRA) showed a reverse accumulation pattern with the highest value at the immature stage (5.10-7.97 μg g-1). Other minor GSLs varied depending on the genotype. Total phenolic and ascorbic acid content increased significantly as maturity progressed in all the genotypes with a relatively higher increment observed in ascorbic acid (65.8%-100.9%) than phenolic content (10.2%-31.2%). Flavonoid content, on the other hand, showed a cultivar- dependent accumulation pattern throughout the developmental stages. However, although antioxidant activity increased with inflorescence development, the increment was higher and lower than in total phenol and ascorbic acid content, respectively. The highest value for GRA (10.57 μg g-1), ascorbic acid (502.3 mg 100 g-1), total phenol (523.7 mg 100 g-1) and antioxidant activities was obtained for ‘09FA-M295’ at the commercial stage. Taken together, these results indicate that phytochemicals in broccoli have a differential accumulation pattern during inflorescence development. Regardless of genotypes and inflorescence developmental stage, total phenolic content showed the highest correlation with antioxidant activity (r = 0.779), followed by ascorbic acid (r = 0.674) and flavonoid content (r = 0.602**), whereas total GSLs showed a nonsignificant correlation with antioxidant activity.
author	Bhandari,Shiva Ram Kwak,Jung-Ho Jo,Jung Su Lee,Jun Gu
author_facet	Bhandari,Shiva Ram Kwak,Jung-Ho Jo,Jung Su Lee,Jun Gu
author_sort	Bhandari,Shiva Ram
title	Changes in phytochemical content and antioxidant activity during inflorescence development in broccoli
title_short	Changes in phytochemical content and antioxidant activity during inflorescence development in broccoli
title_full	Changes in phytochemical content and antioxidant activity during inflorescence development in broccoli
title_fullStr	Changes in phytochemical content and antioxidant activity during inflorescence development in broccoli
title_full_unstemmed	Changes in phytochemical content and antioxidant activity during inflorescence development in broccoli
title_sort	changes in phytochemical content and antioxidant activity during inflorescence development in broccoli
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2019
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392019000100036
work_keys_str_mv	AT bhandarishivaram changesinphytochemicalcontentandantioxidantactivityduringinflorescencedevelopmentinbroccoli AT kwakjungho changesinphytochemicalcontentandantioxidantactivityduringinflorescencedevelopmentinbroccoli AT jojungsu changesinphytochemicalcontentandantioxidantactivityduringinflorescencedevelopmentinbroccoli AT leejungu changesinphytochemicalcontentandantioxidantactivityduringinflorescencedevelopmentinbroccoli
_version_	1714205384480129024

Changes in phytochemical content and antioxidant activity during inflorescence development in broccoli

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