Characterization of grape marc hydrolysates and their antifungal effect against phytopathogenic fungi of agricultural importance

ABSTRACT Winemaking waste contain a high number of bioactive compounds with antimicrobial properties that can be exploited in agriculture. In the present study, hydrolysates from three wine grape (Vitis vinifera L.) marcs were characterized and their antifungal activities against phytopathogenic fun...

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Autores principales:	Tzintzun-Camacho,Olivia, Hernández-Jiménez,Valeria, González-Mendoza,Daniel, Pérez-Pérez,Juan Pedro, Troncoso-Rojas,Rosalba, Durán-Hernández,Dagoberto, Ceceña-Durán,Carlos, Moreno-Cruz,Carlos Francisco
Lenguaje:	English
Publicado:	Instituto de Investigaciones Agropecuarias, INIA 2021
Materias:	Antifungal activity grape marcs hydrolysates phenolic acids phytopathogenic fungi Vitis vinifera.
Acceso en línea:	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392021000200151
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spelling	oai:scielo:S0718-583920210002001512021-05-28Characterization of grape marc hydrolysates and their antifungal effect against phytopathogenic fungi of agricultural importanceTzintzun-Camacho,OliviaHernández-Jiménez,ValeriaGonzález-Mendoza,DanielPérez-Pérez,Juan PedroTroncoso-Rojas,RosalbaDurán-Hernández,DagobertoCeceña-Durán,CarlosMoreno-Cruz,Carlos Francisco Antifungal activity grape marcs hydrolysates phenolic acids phytopathogenic fungi Vitis vinifera. ABSTRACT Winemaking waste contain a high number of bioactive compounds with antimicrobial properties that can be exploited in agriculture. In the present study, hydrolysates from three wine grape (Vitis vinifera L.) marcs were characterized and their antifungal activities against phytopathogenic fungi (Fusarium oxysporum and Alternaria spp.) were evaluated. Wine grape marcs (red, pink and white wine) collected from Ensenada, Baja California, Mexico, were subjected to an acid hydrolysis treatment. Skin hydrolysates of pink and white marcs obtained high concentrations of reducing sugars (5.8 ± 0.1 and 5.5 ± 0.2 g L-1, respectively). Meanwhile, the highest concentration of total sugars was obtained for skin hydrolysates of white marc (9.7 ± 0.08 g L-1). The seed hydrolysates of white marc obtained high concentrations of phenolic compounds (0.52 ± 0.1 mg mL-1). In addition, the highest antioxidant activity was found for skin hydrolysates of red marc (96 ± 0.61%). Results of in vitro antifungal assays clearly indicated a marked inhibition of the mycelial growth and spore viability of F. oxysporum (100% inhibition using red and white hydrolysates) rather than Alternaria spp. (58% inhibition exposed to pink hydrolysates), due to high concentration of phenols. According to HPLC analysis, phenolic acids such as gallic acid, hydroxybenzoic acid, vanillic acid and p-coumaric acid were predominant in the hydrolysates. This study demonstrated that the grape marc hydrolysates exhibit a potential antifungal activity, and highlights that the hydrolysates can be exploited in agriculture as a safe alternative of antifungal agents.info:eu-repo/semantics/openAccessInstituto de Investigaciones Agropecuarias, INIAChilean journal of agricultural research v.81 n.2 20212021-06-01text/htmlhttp://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392021000200151en10.4067/S0718-58392021000200151
institution	Scielo Chile
collection	Scielo Chile
language	English
topic	Antifungal activity grape marcs hydrolysates phenolic acids phytopathogenic fungi Vitis vinifera.
spellingShingle	Antifungal activity grape marcs hydrolysates phenolic acids phytopathogenic fungi Vitis vinifera. Tzintzun-Camacho,Olivia Hernández-Jiménez,Valeria González-Mendoza,Daniel Pérez-Pérez,Juan Pedro Troncoso-Rojas,Rosalba Durán-Hernández,Dagoberto Ceceña-Durán,Carlos Moreno-Cruz,Carlos Francisco Characterization of grape marc hydrolysates and their antifungal effect against phytopathogenic fungi of agricultural importance
description	ABSTRACT Winemaking waste contain a high number of bioactive compounds with antimicrobial properties that can be exploited in agriculture. In the present study, hydrolysates from three wine grape (Vitis vinifera L.) marcs were characterized and their antifungal activities against phytopathogenic fungi (Fusarium oxysporum and Alternaria spp.) were evaluated. Wine grape marcs (red, pink and white wine) collected from Ensenada, Baja California, Mexico, were subjected to an acid hydrolysis treatment. Skin hydrolysates of pink and white marcs obtained high concentrations of reducing sugars (5.8 ± 0.1 and 5.5 ± 0.2 g L-1, respectively). Meanwhile, the highest concentration of total sugars was obtained for skin hydrolysates of white marc (9.7 ± 0.08 g L-1). The seed hydrolysates of white marc obtained high concentrations of phenolic compounds (0.52 ± 0.1 mg mL-1). In addition, the highest antioxidant activity was found for skin hydrolysates of red marc (96 ± 0.61%). Results of in vitro antifungal assays clearly indicated a marked inhibition of the mycelial growth and spore viability of F. oxysporum (100% inhibition using red and white hydrolysates) rather than Alternaria spp. (58% inhibition exposed to pink hydrolysates), due to high concentration of phenols. According to HPLC analysis, phenolic acids such as gallic acid, hydroxybenzoic acid, vanillic acid and p-coumaric acid were predominant in the hydrolysates. This study demonstrated that the grape marc hydrolysates exhibit a potential antifungal activity, and highlights that the hydrolysates can be exploited in agriculture as a safe alternative of antifungal agents.
author	Tzintzun-Camacho,Olivia Hernández-Jiménez,Valeria González-Mendoza,Daniel Pérez-Pérez,Juan Pedro Troncoso-Rojas,Rosalba Durán-Hernández,Dagoberto Ceceña-Durán,Carlos Moreno-Cruz,Carlos Francisco
author_facet	Tzintzun-Camacho,Olivia Hernández-Jiménez,Valeria González-Mendoza,Daniel Pérez-Pérez,Juan Pedro Troncoso-Rojas,Rosalba Durán-Hernández,Dagoberto Ceceña-Durán,Carlos Moreno-Cruz,Carlos Francisco
author_sort	Tzintzun-Camacho,Olivia
title	Characterization of grape marc hydrolysates and their antifungal effect against phytopathogenic fungi of agricultural importance
title_short	Characterization of grape marc hydrolysates and their antifungal effect against phytopathogenic fungi of agricultural importance
title_full	Characterization of grape marc hydrolysates and their antifungal effect against phytopathogenic fungi of agricultural importance
title_fullStr	Characterization of grape marc hydrolysates and their antifungal effect against phytopathogenic fungi of agricultural importance
title_full_unstemmed	Characterization of grape marc hydrolysates and their antifungal effect against phytopathogenic fungi of agricultural importance
title_sort	characterization of grape marc hydrolysates and their antifungal effect against phytopathogenic fungi of agricultural importance
publisher	Instituto de Investigaciones Agropecuarias, INIA
publishDate	2021
url	http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392021000200151
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Characterization of grape marc hydrolysates and their antifungal effect against phytopathogenic fungi of agricultural importance

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