Developmental adaptation of central nervous system to extremely high acetylcholine levels.

Acetylcholinesterase (AChE) is a key enzyme in termination of fast cholinergic transmission. In brain, acetylcholine (ACh) is produced by cholinergic neurons and released in extracellular space where it is cleaved by AChE anchored by protein PRiMA. Recently, we showed that the lack of AChE in brain...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Vladimir Farar, Anna Hrabovska, Eric Krejci, Jaromir Myslivecek
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/12516a657e744683b0867fdeaeca8bd6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:12516a657e744683b0867fdeaeca8bd6
record_format	dspace
spelling	oai:doaj.org-article:12516a657e744683b0867fdeaeca8bd62021-11-18T07:38:32ZDevelopmental adaptation of central nervous system to extremely high acetylcholine levels.1932-620310.1371/journal.pone.0068265https://doaj.org/article/12516a657e744683b0867fdeaeca8bd62013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23861875/?tool=EBIhttps://doaj.org/toc/1932-6203Acetylcholinesterase (AChE) is a key enzyme in termination of fast cholinergic transmission. In brain, acetylcholine (ACh) is produced by cholinergic neurons and released in extracellular space where it is cleaved by AChE anchored by protein PRiMA. Recently, we showed that the lack of AChE in brain of PRiMA knock-out (KO) mouse increased ACh levels 200-300 times. The PRiMA KO mice adapt nearly completely by the reduction of muscarinic receptor (MR) density. Here we investigated changes in MR density, AChE, butyrylcholinesterase (BChE) activity in brain in order to determine developmental period responsible for such adaptation. Brains were studied at embryonal day 18.5 and postnatal days (pd) 0, 9, 30, 120, and 425. We found that the AChE activity in PRiMA KO mice remained very low at all studied ages while in wild type (WT) mice it gradually increased till pd120. BChE activity in WT mice gradually decreased until pd9 and then increased by pd120, it continually decreased in KO mice till pd30 and remained unchanged thereafter. MR number increased in WT mice till pd120 and then became stable. Similarly, MR increased in PRiMA KO mice till pd30 and then remained stable, but the maximal level reached is approximately 50% of WT mice. Therefore, we provide the evidence that adaptive changes in MR happen up to pd30. This is new phenomenon that could contribute to the explanation of survival and nearly unchanged phenotype of PRiMA KO mice.Vladimir FararAnna HrabovskaEric KrejciJaromir MyslivecekPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 7, p e68265 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Vladimir Farar Anna Hrabovska Eric Krejci Jaromir Myslivecek Developmental adaptation of central nervous system to extremely high acetylcholine levels.
description	Acetylcholinesterase (AChE) is a key enzyme in termination of fast cholinergic transmission. In brain, acetylcholine (ACh) is produced by cholinergic neurons and released in extracellular space where it is cleaved by AChE anchored by protein PRiMA. Recently, we showed that the lack of AChE in brain of PRiMA knock-out (KO) mouse increased ACh levels 200-300 times. The PRiMA KO mice adapt nearly completely by the reduction of muscarinic receptor (MR) density. Here we investigated changes in MR density, AChE, butyrylcholinesterase (BChE) activity in brain in order to determine developmental period responsible for such adaptation. Brains were studied at embryonal day 18.5 and postnatal days (pd) 0, 9, 30, 120, and 425. We found that the AChE activity in PRiMA KO mice remained very low at all studied ages while in wild type (WT) mice it gradually increased till pd120. BChE activity in WT mice gradually decreased until pd9 and then increased by pd120, it continually decreased in KO mice till pd30 and remained unchanged thereafter. MR number increased in WT mice till pd120 and then became stable. Similarly, MR increased in PRiMA KO mice till pd30 and then remained stable, but the maximal level reached is approximately 50% of WT mice. Therefore, we provide the evidence that adaptive changes in MR happen up to pd30. This is new phenomenon that could contribute to the explanation of survival and nearly unchanged phenotype of PRiMA KO mice.
format	article
author	Vladimir Farar Anna Hrabovska Eric Krejci Jaromir Myslivecek
author_facet	Vladimir Farar Anna Hrabovska Eric Krejci Jaromir Myslivecek
author_sort	Vladimir Farar
title	Developmental adaptation of central nervous system to extremely high acetylcholine levels.
title_short	Developmental adaptation of central nervous system to extremely high acetylcholine levels.
title_full	Developmental adaptation of central nervous system to extremely high acetylcholine levels.
title_fullStr	Developmental adaptation of central nervous system to extremely high acetylcholine levels.
title_full_unstemmed	Developmental adaptation of central nervous system to extremely high acetylcholine levels.
title_sort	developmental adaptation of central nervous system to extremely high acetylcholine levels.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/12516a657e744683b0867fdeaeca8bd6
work_keys_str_mv	AT vladimirfarar developmentaladaptationofcentralnervoussystemtoextremelyhighacetylcholinelevels AT annahrabovska developmentaladaptationofcentralnervoussystemtoextremelyhighacetylcholinelevels AT erickrejci developmentaladaptationofcentralnervoussystemtoextremelyhighacetylcholinelevels AT jaromirmyslivecek developmentaladaptationofcentralnervoussystemtoextremelyhighacetylcholinelevels
_version_	1718423143260880896

Developmental adaptation of central nervous system to extremely high acetylcholine levels.

Ejemplares similares