Combinatorial CRISPR screen identifies fitness effects of gene paralogues
Systematic screens for synthetic lethal gene pairs represent a powerful approach to define interactions that may be exploited in the clinic. Here, the authors use a dual-guide CRISPR screening approach to screen a curated selection of gene pairs across three cell lines and validate the Fam50a/Fam50b...
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Nature Portfolio
2021
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oai:doaj.org-article:15ae5ef51cdf48aab01435241bfc2c362021-12-02T13:34:49ZCombinatorial CRISPR screen identifies fitness effects of gene paralogues10.1038/s41467-021-21478-92041-1723https://doaj.org/article/15ae5ef51cdf48aab01435241bfc2c362021-02-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-21478-9https://doaj.org/toc/2041-1723Systematic screens for synthetic lethal gene pairs represent a powerful approach to define interactions that may be exploited in the clinic. Here, the authors use a dual-guide CRISPR screening approach to screen a curated selection of gene pairs across three cell lines and validate the Fam50a/Fam50b synthetic lethality was in isogenic cell models as well as in an in vivo setting.Nicola A. ThompsonMarco RanzaniLouise van der WeydenVivek IyerVictoria OffordAlastair DroopFiona BehanEmanuel GonçalvesAnneliese SpeakFrancesco IorioJames HewinsonVictoria HarleHolly RobertsonElizabeth AndersonBeiyuan FuFengtang YangGuido Zagnoli-VieiraPhil ChapmanMartin Del Castillo Velasco-HerreraMathew J. GarnettStephen P. JacksonDavid J. AdamsNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-11 (2021) |
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Science Q Nicola A. Thompson Marco Ranzani Louise van der Weyden Vivek Iyer Victoria Offord Alastair Droop Fiona Behan Emanuel Gonçalves Anneliese Speak Francesco Iorio James Hewinson Victoria Harle Holly Robertson Elizabeth Anderson Beiyuan Fu Fengtang Yang Guido Zagnoli-Vieira Phil Chapman Martin Del Castillo Velasco-Herrera Mathew J. Garnett Stephen P. Jackson David J. Adams Combinatorial CRISPR screen identifies fitness effects of gene paralogues |
description |
Systematic screens for synthetic lethal gene pairs represent a powerful approach to define interactions that may be exploited in the clinic. Here, the authors use a dual-guide CRISPR screening approach to screen a curated selection of gene pairs across three cell lines and validate the Fam50a/Fam50b synthetic lethality was in isogenic cell models as well as in an in vivo setting. |
format |
article |
author |
Nicola A. Thompson Marco Ranzani Louise van der Weyden Vivek Iyer Victoria Offord Alastair Droop Fiona Behan Emanuel Gonçalves Anneliese Speak Francesco Iorio James Hewinson Victoria Harle Holly Robertson Elizabeth Anderson Beiyuan Fu Fengtang Yang Guido Zagnoli-Vieira Phil Chapman Martin Del Castillo Velasco-Herrera Mathew J. Garnett Stephen P. Jackson David J. Adams |
author_facet |
Nicola A. Thompson Marco Ranzani Louise van der Weyden Vivek Iyer Victoria Offord Alastair Droop Fiona Behan Emanuel Gonçalves Anneliese Speak Francesco Iorio James Hewinson Victoria Harle Holly Robertson Elizabeth Anderson Beiyuan Fu Fengtang Yang Guido Zagnoli-Vieira Phil Chapman Martin Del Castillo Velasco-Herrera Mathew J. Garnett Stephen P. Jackson David J. Adams |
author_sort |
Nicola A. Thompson |
title |
Combinatorial CRISPR screen identifies fitness effects of gene paralogues |
title_short |
Combinatorial CRISPR screen identifies fitness effects of gene paralogues |
title_full |
Combinatorial CRISPR screen identifies fitness effects of gene paralogues |
title_fullStr |
Combinatorial CRISPR screen identifies fitness effects of gene paralogues |
title_full_unstemmed |
Combinatorial CRISPR screen identifies fitness effects of gene paralogues |
title_sort |
combinatorial crispr screen identifies fitness effects of gene paralogues |
publisher |
Nature Portfolio |
publishDate |
2021 |
url |
https://doaj.org/article/15ae5ef51cdf48aab01435241bfc2c36 |
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