Combinatorial CRISPR screen identifies fitness effects of gene paralogues

Systematic screens for synthetic lethal gene pairs represent a powerful approach to define interactions that may be exploited in the clinic. Here, the authors use a dual-guide CRISPR screening approach to screen a curated selection of gene pairs across three cell lines and validate the Fam50a/Fam50b...

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Autores principales: Nicola A. Thompson, Marco Ranzani, Louise van der Weyden, Vivek Iyer, Victoria Offord, Alastair Droop, Fiona Behan, Emanuel Gonçalves, Anneliese Speak, Francesco Iorio, James Hewinson, Victoria Harle, Holly Robertson, Elizabeth Anderson, Beiyuan Fu, Fengtang Yang, Guido Zagnoli-Vieira, Phil Chapman, Martin Del Castillo Velasco-Herrera, Mathew J. Garnett, Stephen P. Jackson, David J. Adams
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Lenguaje:EN
Publicado: Nature Portfolio 2021
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Acceso en línea:https://doaj.org/article/15ae5ef51cdf48aab01435241bfc2c36
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spelling oai:doaj.org-article:15ae5ef51cdf48aab01435241bfc2c362021-12-02T13:34:49ZCombinatorial CRISPR screen identifies fitness effects of gene paralogues10.1038/s41467-021-21478-92041-1723https://doaj.org/article/15ae5ef51cdf48aab01435241bfc2c362021-02-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-21478-9https://doaj.org/toc/2041-1723Systematic screens for synthetic lethal gene pairs represent a powerful approach to define interactions that may be exploited in the clinic. Here, the authors use a dual-guide CRISPR screening approach to screen a curated selection of gene pairs across three cell lines and validate the Fam50a/Fam50b synthetic lethality was in isogenic cell models as well as in an in vivo setting.Nicola A. ThompsonMarco RanzaniLouise van der WeydenVivek IyerVictoria OffordAlastair DroopFiona BehanEmanuel GonçalvesAnneliese SpeakFrancesco IorioJames HewinsonVictoria HarleHolly RobertsonElizabeth AndersonBeiyuan FuFengtang YangGuido Zagnoli-VieiraPhil ChapmanMartin Del Castillo Velasco-HerreraMathew J. GarnettStephen P. JacksonDavid J. AdamsNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-11 (2021)
institution DOAJ
collection DOAJ
language EN
topic Science
Q
spellingShingle Science
Q
Nicola A. Thompson
Marco Ranzani
Louise van der Weyden
Vivek Iyer
Victoria Offord
Alastair Droop
Fiona Behan
Emanuel Gonçalves
Anneliese Speak
Francesco Iorio
James Hewinson
Victoria Harle
Holly Robertson
Elizabeth Anderson
Beiyuan Fu
Fengtang Yang
Guido Zagnoli-Vieira
Phil Chapman
Martin Del Castillo Velasco-Herrera
Mathew J. Garnett
Stephen P. Jackson
David J. Adams
Combinatorial CRISPR screen identifies fitness effects of gene paralogues
description Systematic screens for synthetic lethal gene pairs represent a powerful approach to define interactions that may be exploited in the clinic. Here, the authors use a dual-guide CRISPR screening approach to screen a curated selection of gene pairs across three cell lines and validate the Fam50a/Fam50b synthetic lethality was in isogenic cell models as well as in an in vivo setting.
format article
author Nicola A. Thompson
Marco Ranzani
Louise van der Weyden
Vivek Iyer
Victoria Offord
Alastair Droop
Fiona Behan
Emanuel Gonçalves
Anneliese Speak
Francesco Iorio
James Hewinson
Victoria Harle
Holly Robertson
Elizabeth Anderson
Beiyuan Fu
Fengtang Yang
Guido Zagnoli-Vieira
Phil Chapman
Martin Del Castillo Velasco-Herrera
Mathew J. Garnett
Stephen P. Jackson
David J. Adams
author_facet Nicola A. Thompson
Marco Ranzani
Louise van der Weyden
Vivek Iyer
Victoria Offord
Alastair Droop
Fiona Behan
Emanuel Gonçalves
Anneliese Speak
Francesco Iorio
James Hewinson
Victoria Harle
Holly Robertson
Elizabeth Anderson
Beiyuan Fu
Fengtang Yang
Guido Zagnoli-Vieira
Phil Chapman
Martin Del Castillo Velasco-Herrera
Mathew J. Garnett
Stephen P. Jackson
David J. Adams
author_sort Nicola A. Thompson
title Combinatorial CRISPR screen identifies fitness effects of gene paralogues
title_short Combinatorial CRISPR screen identifies fitness effects of gene paralogues
title_full Combinatorial CRISPR screen identifies fitness effects of gene paralogues
title_fullStr Combinatorial CRISPR screen identifies fitness effects of gene paralogues
title_full_unstemmed Combinatorial CRISPR screen identifies fitness effects of gene paralogues
title_sort combinatorial crispr screen identifies fitness effects of gene paralogues
publisher Nature Portfolio
publishDate 2021
url https://doaj.org/article/15ae5ef51cdf48aab01435241bfc2c36
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