Combinatorial CRISPR screen identifies fitness effects of gene paralogues

Systematic screens for synthetic lethal gene pairs represent a powerful approach to define interactions that may be exploited in the clinic. Here, the authors use a dual-guide CRISPR screening approach to screen a curated selection of gene pairs across three cell lines and validate the Fam50a/Fam50b...

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Autores principales:	Nicola A. Thompson, Marco Ranzani, Louise van der Weyden, Vivek Iyer, Victoria Offord, Alastair Droop, Fiona Behan, Emanuel Gonçalves, Anneliese Speak, Francesco Iorio, James Hewinson, Victoria Harle, Holly Robertson, Elizabeth Anderson, Beiyuan Fu, Fengtang Yang, Guido Zagnoli-Vieira, Phil Chapman, Martin Del Castillo Velasco-Herrera, Mathew J. Garnett, Stephen P. Jackson, David J. Adams
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/15ae5ef51cdf48aab01435241bfc2c36
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spelling	oai:doaj.org-article:15ae5ef51cdf48aab01435241bfc2c362021-12-02T13:34:49ZCombinatorial CRISPR screen identifies fitness effects of gene paralogues10.1038/s41467-021-21478-92041-1723https://doaj.org/article/15ae5ef51cdf48aab01435241bfc2c362021-02-01T00:00:00Zhttps://doi.org/10.1038/s41467-021-21478-9https://doaj.org/toc/2041-1723Systematic screens for synthetic lethal gene pairs represent a powerful approach to define interactions that may be exploited in the clinic. Here, the authors use a dual-guide CRISPR screening approach to screen a curated selection of gene pairs across three cell lines and validate the Fam50a/Fam50b synthetic lethality was in isogenic cell models as well as in an in vivo setting.Nicola A. ThompsonMarco RanzaniLouise van der WeydenVivek IyerVictoria OffordAlastair DroopFiona BehanEmanuel GonçalvesAnneliese SpeakFrancesco IorioJames HewinsonVictoria HarleHolly RobertsonElizabeth AndersonBeiyuan FuFengtang YangGuido Zagnoli-VieiraPhil ChapmanMartin Del Castillo Velasco-HerreraMathew J. GarnettStephen P. JacksonDavid J. AdamsNature PortfolioarticleScienceQENNature Communications, Vol 12, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Nicola A. Thompson Marco Ranzani Louise van der Weyden Vivek Iyer Victoria Offord Alastair Droop Fiona Behan Emanuel Gonçalves Anneliese Speak Francesco Iorio James Hewinson Victoria Harle Holly Robertson Elizabeth Anderson Beiyuan Fu Fengtang Yang Guido Zagnoli-Vieira Phil Chapman Martin Del Castillo Velasco-Herrera Mathew J. Garnett Stephen P. Jackson David J. Adams Combinatorial CRISPR screen identifies fitness effects of gene paralogues
description	Systematic screens for synthetic lethal gene pairs represent a powerful approach to define interactions that may be exploited in the clinic. Here, the authors use a dual-guide CRISPR screening approach to screen a curated selection of gene pairs across three cell lines and validate the Fam50a/Fam50b synthetic lethality was in isogenic cell models as well as in an in vivo setting.
format	article
author	Nicola A. Thompson Marco Ranzani Louise van der Weyden Vivek Iyer Victoria Offord Alastair Droop Fiona Behan Emanuel Gonçalves Anneliese Speak Francesco Iorio James Hewinson Victoria Harle Holly Robertson Elizabeth Anderson Beiyuan Fu Fengtang Yang Guido Zagnoli-Vieira Phil Chapman Martin Del Castillo Velasco-Herrera Mathew J. Garnett Stephen P. Jackson David J. Adams
author_facet	Nicola A. Thompson Marco Ranzani Louise van der Weyden Vivek Iyer Victoria Offord Alastair Droop Fiona Behan Emanuel Gonçalves Anneliese Speak Francesco Iorio James Hewinson Victoria Harle Holly Robertson Elizabeth Anderson Beiyuan Fu Fengtang Yang Guido Zagnoli-Vieira Phil Chapman Martin Del Castillo Velasco-Herrera Mathew J. Garnett Stephen P. Jackson David J. Adams
author_sort	Nicola A. Thompson
title	Combinatorial CRISPR screen identifies fitness effects of gene paralogues
title_short	Combinatorial CRISPR screen identifies fitness effects of gene paralogues
title_full	Combinatorial CRISPR screen identifies fitness effects of gene paralogues
title_fullStr	Combinatorial CRISPR screen identifies fitness effects of gene paralogues
title_full_unstemmed	Combinatorial CRISPR screen identifies fitness effects of gene paralogues
title_sort	combinatorial crispr screen identifies fitness effects of gene paralogues
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/15ae5ef51cdf48aab01435241bfc2c36
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Combinatorial CRISPR screen identifies fitness effects of gene paralogues

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