Characteristics of lactic acid bacteria isolated from palm sugar from West Sumatra, Indonesia and their potential as a probiotic

Abstract. Ramadhanti N, Melia S, Hellyward J, Purwati E. 2021. Characteristics of lactic acid bacteria isolated from palm sugar from West Sumatra, Indonesia and their potential as a probiotic. Biodiversitas 22: 2610-2616. Palm sugar is one of the sugar products from palm sap processing. It is made b...

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Autores principales:	NURAZIZAH RAMADHANTI, SRI MELIA, JAMES HELLYWARD, ENDANG PURWATI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2021
Materias:	16s rrna lactic acid bacteria lactobacillus fermentum strain 1743 palm sugar probiotic Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/16101adc83184c1da5ab88fc5b02cf15
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spelling	oai:doaj.org-article:16101adc83184c1da5ab88fc5b02cf152021-11-22T12:13:35ZCharacteristics of lactic acid bacteria isolated from palm sugar from West Sumatra, Indonesia and their potential as a probiotic1412-033X2085-472210.13057/biodiv/d220520https://doaj.org/article/16101adc83184c1da5ab88fc5b02cf152021-04-01T00:00:00Zhttps://smujo.id/biodiv/article/view/8214https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Ramadhanti N, Melia S, Hellyward J, Purwati E. 2021. Characteristics of lactic acid bacteria isolated from palm sugar from West Sumatra, Indonesia and their potential as a probiotic. Biodiversitas 22: 2610-2616. Palm sugar is one of the sugar products from palm sap processing. It is made by reducing the water content in the sap to certain water content and is used as a natural sweetener. This study used palm sugar from 3 producers, i.e. Marapalam Gula Semut (MGS), Tetesan Gula Semut (TGS) and Sungayang Gula Semut (SGS) in Sungayang, West Sumatra, Indonesia. This study aimed to isolate and identify lactic acid bacteria (LAB) from palm sugar, potentially as a probiotic, using the 16S rRNA method. The probiotic potential of bacteria was selected based on morphology, biochemical properties, resistance to acidic pH 3 and 0.3% bile salts, antimicrobial activity against pathogenic bacteria. The results showed that lactic acid bacteria isolated from palm sugar are Gram-positive rod-shaped (Bassil), negative catalase, and homofermentative. The total colonies of lactic acid bacteria ranged from 50 x 107 cfu/g - 117 x 107 cfu/g. The MGS lactic acid bacteria had 74.71% viability resistance in acidic pH 3, 65.52% viability against bile salts (0.3% ox gall). The diameter of inhibition against Escherichia coli O157 was 18.20 mm, Propionibacterium acnes (18.00 mm), Acinetobacter baumannii (14.19 mm), and Listeria monocytogenes (16.35 mm). The phylogenetic tree based on 16S rRNA showed that the MGS isolate has a similarity of 99.92% to Lactobacillus fermentum strain 1743. It can be concluded that Lactobacillus fermentum strain 1743 isolated from palm sugar has potential as a probiotic.NURAZIZAH RAMADHANTISRI MELIAJAMES HELLYWARDENDANG PURWATIMBI & UNS Soloarticle16s rrnalactic acid bacterialactobacillus fermentum strain 1743palm sugarprobioticBiology (General)QH301-705.5ENBiodiversitas, Vol 22, Iss 5 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	16s rrna lactic acid bacteria lactobacillus fermentum strain 1743 palm sugar probiotic Biology (General) QH301-705.5
spellingShingle	16s rrna lactic acid bacteria lactobacillus fermentum strain 1743 palm sugar probiotic Biology (General) QH301-705.5 NURAZIZAH RAMADHANTI SRI MELIA JAMES HELLYWARD ENDANG PURWATI Characteristics of lactic acid bacteria isolated from palm sugar from West Sumatra, Indonesia and their potential as a probiotic
description	Abstract. Ramadhanti N, Melia S, Hellyward J, Purwati E. 2021. Characteristics of lactic acid bacteria isolated from palm sugar from West Sumatra, Indonesia and their potential as a probiotic. Biodiversitas 22: 2610-2616. Palm sugar is one of the sugar products from palm sap processing. It is made by reducing the water content in the sap to certain water content and is used as a natural sweetener. This study used palm sugar from 3 producers, i.e. Marapalam Gula Semut (MGS), Tetesan Gula Semut (TGS) and Sungayang Gula Semut (SGS) in Sungayang, West Sumatra, Indonesia. This study aimed to isolate and identify lactic acid bacteria (LAB) from palm sugar, potentially as a probiotic, using the 16S rRNA method. The probiotic potential of bacteria was selected based on morphology, biochemical properties, resistance to acidic pH 3 and 0.3% bile salts, antimicrobial activity against pathogenic bacteria. The results showed that lactic acid bacteria isolated from palm sugar are Gram-positive rod-shaped (Bassil), negative catalase, and homofermentative. The total colonies of lactic acid bacteria ranged from 50 x 107 cfu/g - 117 x 107 cfu/g. The MGS lactic acid bacteria had 74.71% viability resistance in acidic pH 3, 65.52% viability against bile salts (0.3% ox gall). The diameter of inhibition against Escherichia coli O157 was 18.20 mm, Propionibacterium acnes (18.00 mm), Acinetobacter baumannii (14.19 mm), and Listeria monocytogenes (16.35 mm). The phylogenetic tree based on 16S rRNA showed that the MGS isolate has a similarity of 99.92% to Lactobacillus fermentum strain 1743. It can be concluded that Lactobacillus fermentum strain 1743 isolated from palm sugar has potential as a probiotic.
format	article
author	NURAZIZAH RAMADHANTI SRI MELIA JAMES HELLYWARD ENDANG PURWATI
author_facet	NURAZIZAH RAMADHANTI SRI MELIA JAMES HELLYWARD ENDANG PURWATI
author_sort	NURAZIZAH RAMADHANTI
title	Characteristics of lactic acid bacteria isolated from palm sugar from West Sumatra, Indonesia and their potential as a probiotic
title_short	Characteristics of lactic acid bacteria isolated from palm sugar from West Sumatra, Indonesia and their potential as a probiotic
title_full	Characteristics of lactic acid bacteria isolated from palm sugar from West Sumatra, Indonesia and their potential as a probiotic
title_fullStr	Characteristics of lactic acid bacteria isolated from palm sugar from West Sumatra, Indonesia and their potential as a probiotic
title_full_unstemmed	Characteristics of lactic acid bacteria isolated from palm sugar from West Sumatra, Indonesia and their potential as a probiotic
title_sort	characteristics of lactic acid bacteria isolated from palm sugar from west sumatra, indonesia and their potential as a probiotic
publisher	MBI & UNS Solo
publishDate	2021
url	https://doaj.org/article/16101adc83184c1da5ab88fc5b02cf15
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Characteristics of lactic acid bacteria isolated from palm sugar from West Sumatra, Indonesia and their potential as a probiotic

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