Sequestration and Cyanobacterial Diet Preferences in the Opisthobranch Molluscs Dolabrifera nicaraguana and Stylocheilus rickettsi

A multidisciplinary approach was used to assess chemical ecological dietary interactions between marine organisms as a tool to isolate novel ecologically relevant compounds with biotechnological potential. First, laboratory-based feeding preference assays of the sea hare Dolabrifera nicaraguana (pre...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Kasey E. Clark, Angela Capper, Wei-Ting Liu, Amanda M. Fenner, Alejandro Almanza, Gina Della Togna, Liuris Herrera, Timothy Johns, Valerie J. Paul, Pieter C. Dorrestein, Todd L. Capson, Marcy J. Balunas
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	marine chemical ecology sea hares and cyanobacteria cf. Lyngbya sp. (formerly Lyngbya majuscula) Dolabrifera nicaraguana (formerly D. dolabrifera) Stylocheilus rickettsi (formerly S. striatus) bioactive secondary metabolites Science Q General. Including nature conservation, geographical distribution QH1-199.5
Acceso en línea:	https://doaj.org/article/27810dcad59e49959f71378abcc90190
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:27810dcad59e49959f71378abcc90190
record_format	dspace
spelling	oai:doaj.org-article:27810dcad59e49959f71378abcc901902021-11-10T06:20:41ZSequestration and Cyanobacterial Diet Preferences in the Opisthobranch Molluscs Dolabrifera nicaraguana and Stylocheilus rickettsi2296-774510.3389/fmars.2021.766282https://doaj.org/article/27810dcad59e49959f71378abcc901902021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmars.2021.766282/fullhttps://doaj.org/toc/2296-7745A multidisciplinary approach was used to assess chemical ecological dietary interactions between marine organisms as a tool to isolate novel ecologically relevant compounds with biotechnological potential. First, laboratory-based feeding preference assays of the sea hare Dolabrifera nicaraguana (previously known as D. dolabrifera), an anaspidean mollusc, were conducted by simultaneously offering six food options collected from nearby tidal pools in the Coiba National Park in the Tropical Eastern Pacific of Panama. An evaluation of preferred dietary repertoire revealed D. nicaraguana significantly preferred cf. Lyngbya sp. over the cyanobacterium Symploca sp., green alga Chaetomorpha sp., and red alga Spyridia sp. A no-choice feeding assay using cf. Lyngbya sp. or green alga Cladophora sp. supported this finding. Secondly, we conducted bioactivity-guided fractionation using the preferred food source of D. nicaraguana, the ‘hair-like” cf. Lyngbya sp. from which we also isolated and elucidated two new depsipeptide compounds, veraguamide M (1) and veraguamide N (2). Veraguamides M (1) and N (2) showed in vitro activity toward the malaria-causing parasite Plasmodium falciparum with GI50 values of 4.2 and 4.3 μM, respectively, and therapeutic windows of 7.0–8.0 (based on moderate cytotoxicities to mammalian Vero cells with GI50 values of 29.3 and 34.1 μM, respectively). Veraguamide N (2) was also active against Leishmania donovani, the causative agent of visceral leishmaniasis, with a GI50 value of 6.9 μM. We then evaluated sequestration of these new compounds by D. nicaraguana used in the feeding assays and found trace amounts of the dietary sequestered compounds. Finally, we evaluated sequestration of these new compounds by the sea hare Stylocheilus rickettsi (previously known as S. striatus) that were grazing on the cf. Lyngbya sp. used in the feeding assays and found both to be sequestered. This study is the first example whereby compounds with significant activity against tropical parasites have been found in both the sea hare S. rickettsi and its cyanobacterial food source. These results suggest that chemical ecological studies involving sea hares and cyanobacteria continue to provide a diverse source of bioactive compounds with biotechnological potential.Kasey E. ClarkKasey E. ClarkAngela CapperWei-Ting LiuAmanda M. FennerAlejandro AlmanzaAlejandro AlmanzaGina Della TognaLiuris HerreraTimothy JohnsValerie J. PaulPieter C. DorresteinTodd L. CapsonTodd L. CapsonMarcy J. BalunasMarcy J. BalunasMarcy J. BalunasFrontiers Media S.A.articlemarine chemical ecologysea hares and cyanobacteriacf. Lyngbya sp. (formerly Lyngbya majuscula)Dolabrifera nicaraguana (formerly D. dolabrifera)Stylocheilus rickettsi (formerly S. striatus)bioactive secondary metabolitesScienceQGeneral. Including nature conservation, geographical distributionQH1-199.5ENFrontiers in Marine Science, Vol 8 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	marine chemical ecology sea hares and cyanobacteria cf. Lyngbya sp. (formerly Lyngbya majuscula) Dolabrifera nicaraguana (formerly D. dolabrifera) Stylocheilus rickettsi (formerly S. striatus) bioactive secondary metabolites Science Q General. Including nature conservation, geographical distribution QH1-199.5
spellingShingle	marine chemical ecology sea hares and cyanobacteria cf. Lyngbya sp. (formerly Lyngbya majuscula) Dolabrifera nicaraguana (formerly D. dolabrifera) Stylocheilus rickettsi (formerly S. striatus) bioactive secondary metabolites Science Q General. Including nature conservation, geographical distribution QH1-199.5 Kasey E. Clark Kasey E. Clark Angela Capper Wei-Ting Liu Amanda M. Fenner Alejandro Almanza Alejandro Almanza Gina Della Togna Liuris Herrera Timothy Johns Valerie J. Paul Pieter C. Dorrestein Todd L. Capson Todd L. Capson Marcy J. Balunas Marcy J. Balunas Marcy J. Balunas Sequestration and Cyanobacterial Diet Preferences in the Opisthobranch Molluscs Dolabrifera nicaraguana and Stylocheilus rickettsi
description	A multidisciplinary approach was used to assess chemical ecological dietary interactions between marine organisms as a tool to isolate novel ecologically relevant compounds with biotechnological potential. First, laboratory-based feeding preference assays of the sea hare Dolabrifera nicaraguana (previously known as D. dolabrifera), an anaspidean mollusc, were conducted by simultaneously offering six food options collected from nearby tidal pools in the Coiba National Park in the Tropical Eastern Pacific of Panama. An evaluation of preferred dietary repertoire revealed D. nicaraguana significantly preferred cf. Lyngbya sp. over the cyanobacterium Symploca sp., green alga Chaetomorpha sp., and red alga Spyridia sp. A no-choice feeding assay using cf. Lyngbya sp. or green alga Cladophora sp. supported this finding. Secondly, we conducted bioactivity-guided fractionation using the preferred food source of D. nicaraguana, the ‘hair-like” cf. Lyngbya sp. from which we also isolated and elucidated two new depsipeptide compounds, veraguamide M (1) and veraguamide N (2). Veraguamides M (1) and N (2) showed in vitro activity toward the malaria-causing parasite Plasmodium falciparum with GI50 values of 4.2 and 4.3 μM, respectively, and therapeutic windows of 7.0–8.0 (based on moderate cytotoxicities to mammalian Vero cells with GI50 values of 29.3 and 34.1 μM, respectively). Veraguamide N (2) was also active against Leishmania donovani, the causative agent of visceral leishmaniasis, with a GI50 value of 6.9 μM. We then evaluated sequestration of these new compounds by D. nicaraguana used in the feeding assays and found trace amounts of the dietary sequestered compounds. Finally, we evaluated sequestration of these new compounds by the sea hare Stylocheilus rickettsi (previously known as S. striatus) that were grazing on the cf. Lyngbya sp. used in the feeding assays and found both to be sequestered. This study is the first example whereby compounds with significant activity against tropical parasites have been found in both the sea hare S. rickettsi and its cyanobacterial food source. These results suggest that chemical ecological studies involving sea hares and cyanobacteria continue to provide a diverse source of bioactive compounds with biotechnological potential.
format	article
author	Kasey E. Clark Kasey E. Clark Angela Capper Wei-Ting Liu Amanda M. Fenner Alejandro Almanza Alejandro Almanza Gina Della Togna Liuris Herrera Timothy Johns Valerie J. Paul Pieter C. Dorrestein Todd L. Capson Todd L. Capson Marcy J. Balunas Marcy J. Balunas Marcy J. Balunas
author_facet	Kasey E. Clark Kasey E. Clark Angela Capper Wei-Ting Liu Amanda M. Fenner Alejandro Almanza Alejandro Almanza Gina Della Togna Liuris Herrera Timothy Johns Valerie J. Paul Pieter C. Dorrestein Todd L. Capson Todd L. Capson Marcy J. Balunas Marcy J. Balunas Marcy J. Balunas
author_sort	Kasey E. Clark
title	Sequestration and Cyanobacterial Diet Preferences in the Opisthobranch Molluscs Dolabrifera nicaraguana and Stylocheilus rickettsi
title_short	Sequestration and Cyanobacterial Diet Preferences in the Opisthobranch Molluscs Dolabrifera nicaraguana and Stylocheilus rickettsi
title_full	Sequestration and Cyanobacterial Diet Preferences in the Opisthobranch Molluscs Dolabrifera nicaraguana and Stylocheilus rickettsi
title_fullStr	Sequestration and Cyanobacterial Diet Preferences in the Opisthobranch Molluscs Dolabrifera nicaraguana and Stylocheilus rickettsi
title_full_unstemmed	Sequestration and Cyanobacterial Diet Preferences in the Opisthobranch Molluscs Dolabrifera nicaraguana and Stylocheilus rickettsi
title_sort	sequestration and cyanobacterial diet preferences in the opisthobranch molluscs dolabrifera nicaraguana and stylocheilus rickettsi
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/27810dcad59e49959f71378abcc90190
work_keys_str_mv	AT kaseyeclark sequestrationandcyanobacterialdietpreferencesintheopisthobranchmolluscsdolabriferanicaraguanaandstylocheilusrickettsi AT kaseyeclark sequestrationandcyanobacterialdietpreferencesintheopisthobranchmolluscsdolabriferanicaraguanaandstylocheilusrickettsi AT angelacapper sequestrationandcyanobacterialdietpreferencesintheopisthobranchmolluscsdolabriferanicaraguanaandstylocheilusrickettsi AT weitingliu sequestrationandcyanobacterialdietpreferencesintheopisthobranchmolluscsdolabriferanicaraguanaandstylocheilusrickettsi AT amandamfenner sequestrationandcyanobacterialdietpreferencesintheopisthobranchmolluscsdolabriferanicaraguanaandstylocheilusrickettsi AT alejandroalmanza sequestrationandcyanobacterialdietpreferencesintheopisthobranchmolluscsdolabriferanicaraguanaandstylocheilusrickettsi AT alejandroalmanza sequestrationandcyanobacterialdietpreferencesintheopisthobranchmolluscsdolabriferanicaraguanaandstylocheilusrickettsi AT ginadellatogna sequestrationandcyanobacterialdietpreferencesintheopisthobranchmolluscsdolabriferanicaraguanaandstylocheilusrickettsi AT liurisherrera sequestrationandcyanobacterialdietpreferencesintheopisthobranchmolluscsdolabriferanicaraguanaandstylocheilusrickettsi AT timothyjohns sequestrationandcyanobacterialdietpreferencesintheopisthobranchmolluscsdolabriferanicaraguanaandstylocheilusrickettsi AT valeriejpaul sequestrationandcyanobacterialdietpreferencesintheopisthobranchmolluscsdolabriferanicaraguanaandstylocheilusrickettsi AT pietercdorrestein sequestrationandcyanobacterialdietpreferencesintheopisthobranchmolluscsdolabriferanicaraguanaandstylocheilusrickettsi AT toddlcapson sequestrationandcyanobacterialdietpreferencesintheopisthobranchmolluscsdolabriferanicaraguanaandstylocheilusrickettsi AT toddlcapson sequestrationandcyanobacterialdietpreferencesintheopisthobranchmolluscsdolabriferanicaraguanaandstylocheilusrickettsi AT marcyjbalunas sequestrationandcyanobacterialdietpreferencesintheopisthobranchmolluscsdolabriferanicaraguanaandstylocheilusrickettsi AT marcyjbalunas sequestrationandcyanobacterialdietpreferencesintheopisthobranchmolluscsdolabriferanicaraguanaandstylocheilusrickettsi AT marcyjbalunas sequestrationandcyanobacterialdietpreferencesintheopisthobranchmolluscsdolabriferanicaraguanaandstylocheilusrickettsi
_version_	1718440450983985152

Sequestration and Cyanobacterial Diet Preferences in the Opisthobranch Molluscs Dolabrifera nicaraguana and Stylocheilus rickettsi

Ejemplares similares