The Effect of NaCl Concentration and Incubation Time on Oxalate and Total Acid in Fermented Cabbage using Various Microorganisms

As the highest agrocultural product, cabbage (Brassica oleracea var. capitata) remain consider as perishable vegetable and also known to contain an antinutritional compound, called oxalate. The oxalate salts is non-soluble compound in water, and thus settle on human kidney. The preservation and imp...

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Autores principales: Arie Srihardyastutie, Sasangka Prasetyawan, Radhinal Zikri Firdaus, Fidelia Berenice Prayugo, Yolanda Kresmonia, Tinok Dwi Ananda, Alfi Salamah
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Publicado: University of Brawijaya 2021
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spelling oai:doaj.org-article:2a7f2964dc7e40638a623bb89912d6762021-11-24T11:02:12ZThe Effect of NaCl Concentration and Incubation Time on Oxalate and Total Acid in Fermented Cabbage using Various Microorganisms2302-46902541-0733https://doaj.org/article/2a7f2964dc7e40638a623bb89912d6762021-04-01T00:00:00Zhttps://jpacr.ub.ac.id/index.php/jpacr/article/view/568/pdfhttps://doaj.org/toc/2302-4690https://doaj.org/toc/2541-0733As the highest agrocultural product, cabbage (Brassica oleracea var. capitata) remain consider as perishable vegetable and also known to contain an antinutritional compound, called oxalate. The oxalate salts is non-soluble compound in water, and thus settle on human kidney. The preservation and improvement of the nutritional aspect of cabbage can be achieved by fermentation through the enzyme production. Therefore, we study the effect of salt addition and incubation time, as the two important factor in commonly cabbage-based fermentation, combined with someoxalate-degrading-bacteria, which wasL. plantarum, L. plantarum – S. cerevisiae, and A. aceti – S. cerevisiae on the oxalate and total acid level in cabbage fermentation. The fermentation was conducted in submerged system at room temperature (25 – 27 °C) with the NaCl concentration addition ranging from 0 – 5% until 10 days. Those condition was carried out for the three types of the tested cultures. The oxalate and total acid level was measured using the permanganometric and acid base titration, respectively. The results indicated that L. plantarum-induced fermentation combined with 3% NaCl during 10 days gave the lowest oxalate level in the fermented cabbage biomass,which was 0.005 mg/100 g FW. Moreover, these single-cultured fermentation was able to produce the highest total acid level in the brine solution, 1.270% at the 8 days of fermentation. This fermentation serves as an alternative method to improve cabbage consumption.Arie SrihardyastutieSasangka PrasetyawanRadhinal Zikri FirdausFidelia Berenice PrayugoYolanda KresmoniaTinok Dwi AnandaAlfi SalamahUniversity of Brawijayaarticlecabbagefermentationoxalatetotal acidChemistryQD1-999ENJournal of Pure and Applied Chemistry Research, Vol 10, Iss 1, Pp 27-36 (2021)
institution DOAJ
collection DOAJ
language EN
topic cabbage
fermentation
oxalate
total acid
Chemistry
QD1-999
spellingShingle cabbage
fermentation
oxalate
total acid
Chemistry
QD1-999
Arie Srihardyastutie
Sasangka Prasetyawan
Radhinal Zikri Firdaus
Fidelia Berenice Prayugo
Yolanda Kresmonia
Tinok Dwi Ananda
Alfi Salamah
The Effect of NaCl Concentration and Incubation Time on Oxalate and Total Acid in Fermented Cabbage using Various Microorganisms
description As the highest agrocultural product, cabbage (Brassica oleracea var. capitata) remain consider as perishable vegetable and also known to contain an antinutritional compound, called oxalate. The oxalate salts is non-soluble compound in water, and thus settle on human kidney. The preservation and improvement of the nutritional aspect of cabbage can be achieved by fermentation through the enzyme production. Therefore, we study the effect of salt addition and incubation time, as the two important factor in commonly cabbage-based fermentation, combined with someoxalate-degrading-bacteria, which wasL. plantarum, L. plantarum – S. cerevisiae, and A. aceti – S. cerevisiae on the oxalate and total acid level in cabbage fermentation. The fermentation was conducted in submerged system at room temperature (25 – 27 °C) with the NaCl concentration addition ranging from 0 – 5% until 10 days. Those condition was carried out for the three types of the tested cultures. The oxalate and total acid level was measured using the permanganometric and acid base titration, respectively. The results indicated that L. plantarum-induced fermentation combined with 3% NaCl during 10 days gave the lowest oxalate level in the fermented cabbage biomass,which was 0.005 mg/100 g FW. Moreover, these single-cultured fermentation was able to produce the highest total acid level in the brine solution, 1.270% at the 8 days of fermentation. This fermentation serves as an alternative method to improve cabbage consumption.
format article
author Arie Srihardyastutie
Sasangka Prasetyawan
Radhinal Zikri Firdaus
Fidelia Berenice Prayugo
Yolanda Kresmonia
Tinok Dwi Ananda
Alfi Salamah
author_facet Arie Srihardyastutie
Sasangka Prasetyawan
Radhinal Zikri Firdaus
Fidelia Berenice Prayugo
Yolanda Kresmonia
Tinok Dwi Ananda
Alfi Salamah
author_sort Arie Srihardyastutie
title The Effect of NaCl Concentration and Incubation Time on Oxalate and Total Acid in Fermented Cabbage using Various Microorganisms
title_short The Effect of NaCl Concentration and Incubation Time on Oxalate and Total Acid in Fermented Cabbage using Various Microorganisms
title_full The Effect of NaCl Concentration and Incubation Time on Oxalate and Total Acid in Fermented Cabbage using Various Microorganisms
title_fullStr The Effect of NaCl Concentration and Incubation Time on Oxalate and Total Acid in Fermented Cabbage using Various Microorganisms
title_full_unstemmed The Effect of NaCl Concentration and Incubation Time on Oxalate and Total Acid in Fermented Cabbage using Various Microorganisms
title_sort effect of nacl concentration and incubation time on oxalate and total acid in fermented cabbage using various microorganisms
publisher University of Brawijaya
publishDate 2021
url https://doaj.org/article/2a7f2964dc7e40638a623bb89912d676
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